Estrous cycle impacts on dendritic spine plasticity in rat nucleus accumbens core and shell and caudate–putamen

• Published in: Journal of comparative neurology (1911) 2023-05, Vol.531 (7), p.759-774
• Main Authors: Beeson, Anna L. S., Meitzen, John
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Anatomy; Animals; Brain architecture; caudate–putamen; Dendritic plasticity; dendritic spine; Dendritic spines; Dendritic Spines - physiology; Electrophysiology; Estradiol; Estrous Cycle; Estrus cycle; Female; Females; Glutamatergic transmission; Humans; Male; Menstrual cycle; Neostriatum; Neuromodulation; Nucleus Accumbens; Putamen; Rats; Rats, Sprague-Dawley; Sex; sex differences; Synapses
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.25460

An important factor that can modulate neuron properties is sex‐specific hormone fluctuations, including the human menstrual cycle and rat estrous cycle in adult females. Considering the striatal brain regions, the nucleus accumbens (NAc) core, NAc shell, and caudate–putamen (CPu), the estrous cycle has previously been shown to impact relevant behaviors and disorders, neuromodulator action, and medium spiny neuron (MSN) electrophysiology. Whether the estrous cycle impacts MSN dendritic spine attributes has not yet been examined, even though MSN spines and glutamatergic synapse properties are sensitive to exogenously applied estradiol. Thus, we hypothesized that MSN dendritic spine attributes would differ by estrous cycle phase. To test this hypothesis, brains from adult male rats and female rats in diestrus, proestrus AM, proestrus PM, and estrus were processed for Rapid Golgi–Cox staining. MSN dendritic spine density, size, and type were analyzed in the NAc core, NAc shell, and CPu. Overall spine size differed across estrous cycle phases in female NAc core and NAc shell, and spine length differed across estrous cycle phase in NAc shell and CPu. Consistent with previous work, dendritic spine density was increased in the NAc core compared to the NAc shell and CPu, independent of sex and estrous cycle. Spine attributes in all striatal regions did not differ by sex when estrous cycle was disregarded. These results indicate, for the first time, that estrous cycle phase impacts dendritic spine plasticity in striatal regions, providing a neuroanatomical avenue by which sex‐specific hormone fluctuations can impact striatal function and disorders. Dendritic spine size differs between estrous cycle phases within the nucleus accumbens (NAc) core and shell of adult female rats. Spine size was decreased in proestrus PM compared to AM in NAc core (left) and was increased in estrus compared to proestrus PM and diestrus in NAc shell (right).