Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib

• Published in: Journal of clinical immunology 2024-04, Vol.44 (4), p.85-85, Article 85
• Main Authors: Dotta, Laura, Todaro, Francesca, Baronio, Manuela, Giacomelli, Mauro, Pinelli, Marinella, Giambarda, Martina, Brognoli, Beatrice, Greco, Silvia, Rota, Francesca, Cortesi, Manuela, Soresina, Annarosa, Moratto, Daniele, Tomasi, Cesare, Ferraro, Rosalba Monica, Giliani, Silvia, Badolato, Raffaele
• Format: Article
• Language: English
• Subjects: Adult; adults; Annexin V; Apoptosis; Autoimmunity; Biomedical and Life Sciences; Biomedicine; Candidiasis; Candidiasis, Chronic Mucocutaneous - drug therapy; Candidiasis, Chronic Mucocutaneous - genetics; Chronic mucocutaneous candidiasis; Flow cytometry; fungi; Gain of Function Mutation; genes; Humans; Immunology; Infectious Diseases; Inhibitor drugs; Interferons; Internal Medicine; Janus Kinase Inhibitors - therapeutic use; Lymphocytes T; Lymphopenia; Malignancy; Medical Microbiology; Mitogens; Morbidity; Nitriles; Patients; phenotype; Phenotypes; Phosphorylation; propidium; Propidium iodide; Pyrazoles; Pyrimidines; risk; signal transduction; Stat1 protein; STAT1 Transcription Factor - metabolism; T-lymphocytes; therapeutics; Thrombocytopenia; transactivators; α-Interferon
• ISSN: 0271-9142; 1573-2592; 1573-2592
• DOI: 10.1007/s10875-024-01684-y

Introduction The signal transducer and activator of transcription (STAT1) gain-of-function (GOF) syndrome accounts for most cases of chronic mucocutaneous candidiasis but is characterized by a broader clinical phenotype that may include bacterial, viral, or invasive fungal infections, autoimmunity, autoinflammatory manifestations, vascular complications, or malignancies. The severity of lymphopenia may vary and influence the infectious morbidity. Methods In our cohort of seven STAT1-GOF patients, we investigated the mechanisms that may determine T lymphopenia, we characterized the interferon gene signature (IGS) and analyzed the effect of ruxolitinib in reverting the immune dysregulation. Results STAT1-GOF patients exhibited increased T lymphocyte apoptosis that was significantly augmented in both resting conditions and following stimulation with mitogens and IFNα, as evaluated by flow cytometry by Annexin V/ Propidium iodide assay. The JAK inhibitor ruxolitinib significantly reduced the IFNα-induced hyperphosphorylation of STAT1 and reverted the stimulation-induced T-cell apoptosis, in vitro. In two adult STAT1-GOF patients, the JAKinib treatment ameliorated chronic mucocutaneous candidiasis and lymphopenia. Most STAT1-GOF patients, particularly those who had autoimmunity, presented increased IGS that significantly decreased in the two patients during ruxolitinib treatment. Conclusion In STAT1-GOF patients, T lymphocyte apoptosis is increased, and T lymphopenia may determine higher risk of severe infections. The JAKinib target therapy should be evaluated to treat severe chronic candidiasis and lymphopenia, and to downregulate the IFNs in patients with autoinflammatory or autoimmune manifestations.