Texaphyrin-Based Calcium Sensor for Multimodal Imaging

The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccess...

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Bibliographic Details
Published in:ACS sensors 2023-10, Vol.8 (10), p.3855-3861
Main Authors: Thiabaud, Grégory D., Schwalm, Miriam, Sen, Sajal, Barandov, Ali, Simon, Jacob, Harvey, Peter, Spanoudaki, Virginia, Müller, Peter, Sessler, Jonathan L., Jasanoff, Alan
Format: Article
Language:English
Subjects:
Calcium
Magnetic Resonance Imaging - methods
Multimodal Imaging - methods
Porphyrins
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Summary:The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccessible to optical techniques. To meet this need, we synthesized molecular probes that couple calcium-binding moieties to lanthanide texaphyrins, resulting in complexes endowed with a diverse complement of magnetic and photophysical properties. We show that the probes permit intracellular calcium levels to be assessed by fluorescence, photoacoustic, and magnetic resonance imaging modalities and that they are detectable by multimodal imaging in brain tissue. This work thus establishes a route for monitoring signaling processes over a range of spatial and temporal scales.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.3c01387