Specific interaction between Group B Streptococcus CC17 hypervirulent clone and phagocytes

• Published in: Infection and immunity 2024-04, Vol.92 (4), p.e0006224
• Main Authors: Bourrel, Anne-Sophie, Picart, Amandine, Fernandez, Jose-Carlos, Hays, Constantin, Mignon, Virginie, Saubaméa, Bruno, Poyart, Claire, Fouet, Agnès, Tazi, Asmaa, Guignot, Julie
• Format: Article
• Language: English
• Subjects: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions; Clone Cells; Host-Microbial Interactions; Humans; Infant, Newborn; Macrophages; Meningitis; Streptococcal Infections - microbiology; Streptococcus agalactiae
• ISSN: 0019-9567; 1098-5522; 1098-5522
• DOI: 10.1128/iai.00062-24

also named Group B (GBS) is the most significant pathogen causing invasive infections, such as bacteremia and meningitis, in neonates. Worldwide epidemiological studies have shown that a particular clonal complex (CC) of capsular serotype III, the CC17, is strongly associated with meningitis in neonates and is therefore, designated as the hypervirulent clone. Macrophages are a permissive niche for intracellular bacteria of all GBS clones. In this study, we deciphered the specific interaction of GBS CC17 strains with macrophages. Our study revealed that CC17 strains are phagocytosed at a higher rate than GBS non-CC17 strains by human monocytes and macrophages both in cellular models and in primary cells. CC17-enhanced phagocytosis is due to an initial enhanced-attachment step to macrophages mediated by the CC17-specific surface protein HvgA and the PI-2b pilus (Spb1). We showed that two different inhibitors of scavenger receptors (fucoidan and poly(I)) specifically inhibited CC17 adhesion and phagocytosis while not affecting those of non-CC17 strains. Once phagocytosed, both CC17 and non-CC17 strains remained in a LAMP-1 positive vacuole that ultimately fuses with lysosomes where they can survive at similar rates. Finally, both strains displayed a basal egress which occurs independently from actin and microtubule networks. Our findings provide new insights into the interplay between the hypervirulent GBS CC17 and major players of the host's innate immune response. This enhanced adhesion, leading to increased phagocytosis, could reflect a peculiar capacity of the CC17 lineage to subvert the host immune defenses, establish a niche for persistence or disseminate.