Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with Follicular Fluid Inflammatory Markers

• Published in: Avicenna journal of medical biotechnology 2024, Vol.16 (1), p.16-28
• Main Authors: Sarvari, Ali, Niasari-Naslaji, Amir, Shirazi, Abolfazl, Heidari, Banafsheh, Boroujeni, Sara Borjian, Moradi, Mohammad Hossein, Naderi, Mohammad-Mahdi, Behzadi, Bahareh, Mehrazar, Mohammad-Mahdi, Dehghan, Mohammad Mehdi
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2008-2835; 2008-4625
• DOI: 10.18502/ajmb.v16i1.14167

Repeated Ovum Pick Up (OPU) could have a detrimental effect on ovarian function, reducing Embryo Production (IVEP). The present study examined the therapeutic effect of adipose-derived Mesenchymal Stem Cells (MSCs) or its Conditioned Medium (ConM) on ovarian trauma following repeated OPU. Resolvin E1 (RvE1) and Interleukin-12 (IL-12) were investigated as biomarkers. Jersey heifers (n=8) experienced 11 OPU sessions including 5 pre-treatment and 6 treatment sessions. Heifers received intra-ovarian administration of MSCs or ConM (right ovary) and Dulbecco's Modified Phosphate Buffer Saline (DMPBS; left ovary) after OPU in sessions 5 and 8 and 2 weeks after session 11. The concentrations of RvE1 and IL-12 in follicular fluid was evaluated on sessions 1, 5, 6, 9, and 4 weeks after session 11. Following each OPU session, the IVEP parameters were recorded. Intra-ovarian administration of MSCs, ConM, and DMPBS did not affect IVEP parameters (p>0.05). The concentration of IL-12 in follicular fluid increased at the last session of pre-treatment (Session 5; p0.05). However, RvE1 remained relatively high during the pre-treatment and decreased toward the end of treatment period (p