PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells

High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5′ untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode...

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Published in:Doklady. Biochemistry and biophysics 2024-04, Vol.515 (1), p.41-47
Main Authors: Shepelev, N. M., Kurochkina, A. O., Dontsova, O. A., Rubtsova, M. P.
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
DNA Repair Enzymes - genetics
DNA Repair Enzymes - metabolism
Humans
Life Sciences
mRNA
Nuclear Proteins - metabolism
Open Reading Frames
Protein Biosynthesis
Ribosomes - metabolism
RNA Splicing Factors - genetics
RNA Splicing Factors - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5′ untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode a small functional protein or microprotein. In this work, we showed that the 5′ untranslated region of the PRPF19 mRNA encodes an upstream ORF that is translated in human cells. Inactivation of this upstream ORF reduces the viability of human cells.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672923700722