Immunotherapy with SLPI over-expressing mammary tumor cells decreases tumor growth

We have demonstrated previously that the inoculation of murine mammary tumor cells genetically modified to express high levels of secretory leukocyte protease inhibitor (2C1) do not develop tumors in immunocompetent mice and these cells are more prone to apoptosis than control cells. The aim of the...

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Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 2011-06, Vol.60 (6), p.895-900
Main Authors: Amiano, Nicolás, Reiteri, R. Macarena, Costa, María J., Tateosian, Nancy, Chuluyan, H. Eduardo
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Apoptosis
Biological and medical sciences
Cancer
Cancer Research
Cell Growth Processes - immunology
Female
Immunology
Immunotherapy
Immunotherapy, Adoptive - methods
Leukocytes
Mammary Neoplasms, Experimental - genetics
Mammary Neoplasms, Experimental - immunology
Mammary Neoplasms, Experimental - pathology
Mammary Neoplasms, Experimental - therapy
Medical sciences
Medicine
Medicine & Public Health
Mice
Mice, Inbred BALB C
Mice, Nude
Oncology
Pharmacology. Drug treatments
Secretory Leukocyte Peptidase Inhibitor - genetics
Secretory Leukocyte Peptidase Inhibitor - immunology
Short Communication
Statistical analysis
Transfection
Tumors
Vaccines
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Summary:We have demonstrated previously that the inoculation of murine mammary tumor cells genetically modified to express high levels of secretory leukocyte protease inhibitor (2C1) do not develop tumors in immunocompetent mice and these cells are more prone to apoptosis than control cells. The aim of the present study was to evaluate the role of the adaptive immune response in the lack of tumor growth of 2C1 cells and the possibility of using these cells for immunotherapy. The s.c. administration of mock transfected F3II cells induces tumor in BALB/c and Nude mice. However, the inoculation of 2C1 cells develops tumor in Nude but not in BALB/c mice. The inoculation of mock transfected F3II cells to 2C1 immunized BALB/c mice by repeated administration of 2C1 cells (once a week for 3 weeks) developed significantly smaller tumors than those observed in non-immunized mice. Remarkably, survival of tumor-bearing immunized mice was higher than non-immunized animals. Herein, we demonstrate that an immunotherapy with SLPI over-expressing non-irradiated tumor cells which do not develop tumor in immunocompetent mice, partially restrain the tumor growth induced by F3II cells and increase the survival of the mice.
ISSN:0340-7004
1432-0851
DOI:10.1007/s00262-011-1018-2