Molecular and immunological evaluation of the expression of cancer/testis gene products in human colorectal cancer

Tumor-specific gene products, such as cancer/testis (CT) antigens, constitute promising targets for the development of T cell vaccines. Whereas CT antigens are frequently expressed in melanoma, their expression in colorectal cancers (CRC) remains poorly characterized. Here, we have studied the expre...

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Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 2007-06, Vol.56 (6), p.839-847
Main Authors: Alves, Pedro M S, Lévy, Nicole, Bouzourene, Hanifa, Viatte, Sébastien, Bricard, Gabriel, Ayyoub, Maha, Vuilleumier, Henri, Givel, Jean-Claude R, Halkic, Nermin, Speiser, Daniel E, Romero, Pedro, Lévy, Frédéric
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - biosynthesis
Antigens, Neoplasm - genetics
Antigens, Neoplasm - immunology
CD8-Positive T-Lymphocytes - immunology
Colorectal Neoplasms - genetics
Colorectal Neoplasms - immunology
Colorectal Neoplasms - metabolism
Gene Expression
Gene Expression Profiling
Humans
Immunohistochemistry
Liver Neoplasms - metabolism
Liver Neoplasms - secondary
Male
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
Neoplasm Proteins - immunology
Original
Reverse Transcriptase Polymerase Chain Reaction
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Summary:Tumor-specific gene products, such as cancer/testis (CT) antigens, constitute promising targets for the development of T cell vaccines. Whereas CT antigens are frequently expressed in melanoma, their expression in colorectal cancers (CRC) remains poorly characterized. Here, we have studied the expression of the CT antigens MAGE-A3, MAGE-A4, MAGE-A10, NY-ESO-1 and SSX2 in CRC because of the presence of well-described HLA-A2-restricted epitopes in their sequences. Our analyses of 41 primary CRC and 14 metastatic liver lesions confirmed the low frequency of expression of these CT antigens. No increased expression frequencies were observed in metastatic tumors compared to primary tumors. Histological analyses of CRC samples revealed heterogeneous expression of individual CT antigens. Finally, evidence of a naturally acquired CT antigen-specific CD8(+) T cell response could be demonstrated. These results show that the expression of CT antigens in a subset of CRC patients induces readily detectable T cell responses.
ISSN:0340-7004
1432-0851
1365-2567
DOI:10.1007/s00262-006-0228-5