Engineering a nanoantibiotic system displaying dual mechanism of action

• Published in: Proceedings of the National Academy of Sciences - PNAS 2024-04, Vol.121 (16), p.e2321498121
• Main Authors: Xing, Huihua, de Campos, Luana Janaína, Pereira, Aramis Jose, Fiora, Maria Mercedes, Aguiar-Alves, Fabio, Tagliazucchi, Mario, Conda-Sheridan, Martin
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents; Antimicrobial activity; Antimicrobial agents; Bacteria; Biocompatibility; Biological Sciences; Biomimetics; Cytotoxicity; Effectiveness; Encapsulation; Fluorescence; Gram-Negative Bacteria; Gram-Positive Bacteria; In vivo methods and tests; Inoculum; Larvae; Membranes; Microscopy; Molecular theory; Morphology; Nanostructure; Nanostructures - chemistry; NMR; Nuclear magnetic resonance; Pharmacology; Physical Sciences; Polymyxin B; Protease inhibitors; Proteinase inhibitors; Self-assembly; Transmission electron microscopy
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2321498121

In recent decades, peptide amphiphiles (PAs) have established themselves as promising self-assembling bioinspired materials in a wide range of medical fields. Herein, we report a dual-therapeutic system constituted by an antimicrobial PA and a cylindrical protease inhibitor (LJC) to achieve broad antimicrobial spectrum and to enhance therapeutic efficacy. We studied two strategies: PA-LJC nanostructures ( ) and PA nanostructures + free LJC ( ). Computational modeling using a molecular theory for amphiphile self-assembly captures and explains the morphology of PA-LJC nanostructures and the location of encapsulated LJC in agreement with transmission electron microscopy and two-dimensional (2D) NMR observations. The morphology and release profile of PA-LJC assemblies are strongly correlated to the PA:LJC ratio: high LJC loading induces an initial burst release. We then evaluated the antimicrobial activity of our nanosystems toward gram-positive and gram-negative bacteria. We found that the broadens the spectrum of LJC, reduces the therapeutic concentrations of both agents, and is not impacted by the inoculum effect. Further, the provides additional benefits including bypassing water solubility limitations of LJC and modulating the release of this molecule. The different properties of PA-LJC nanostructures results in different killing profiles, and reduced cytotoxicity and hemolytic activity. Meanwhile, details in membrane alterations caused by each strategy were revealed by various microscopy and fluorescent techniques. Last, in vivo studies in larvae treated by the strategy showed better antimicrobial efficacy than polymyxin B. Collectively, this study established a multifunctional platform using a versatile PA to act as an antibiotic, membrane-penetrating assistant, and slow-release delivery vehicle.