Accumulation of CD45RO(+) cells in peritoneal carcinomatous fluid favours survival of ovarian carcinoma patients

• Published in: Cancer Immunology, Immunotherapy Immunotherapy, 2002-11, Vol.51 (9), p.513-519
• Main Authors: Kryczek, Ilona, Gryboś, Marian, Dlubek, Dorota, Klimczak, Aleksandra, Rabczyński, Jerzy, Lange, Andrzej
• Format: Article
• Language: English
• Subjects: Age Factors; Ascitic Fluid - metabolism; Cancer therapies; Carcinoma - metabolism; Chemokine CXCL10; Chemokines, CXC - biosynthesis; Chemotherapy; Cisplatin - therapeutic use; Cyclophosphamide - therapeutic use; Cytokines; Doxorubicin - therapeutic use; Female; Flow Cytometry; Humans; Immunohistochemistry; Immunology; Interleukin-10 - biosynthesis; Interleukin-10 - metabolism; Interleukin-2 - biosynthesis; Interleukin-6 - biosynthesis; Interleukin-6 - metabolism; Ki-67 Antigen - biosynthesis; Leukocyte Common Antigens - biosynthesis; Leukocytes; Lymphocytes; Monoclonal antibodies; Original; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - mortality; Ovaries; Peritoneum - metabolism; Proteins; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA, Messenger - metabolism; Survival analysis; Time Factors; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0340-7004; 1432-0851
• DOI: 10.1007/s00262-002-0307-1

In 44 patients with advanced ovarian carcinoma (OC) a fraction of CD45RO(+) lymphocytes in the blood and peritoneal carcinomatous fluid (PCF) was investigated. Thirty-one patients received cisplatinum with cyclophosphamide +/- doxorubicin. This group was followed from 2.2 to 9 years (mean: 45 months). In 23 out of 31 patients, the percentage of CD45RO(+) lymphocytes was higher in the PCF than in the blood samples. Patients with these higher lymphocyte levels experienced longer survival than those who did not show any excess of CD45RO(+) lymphocytes in PCF ( P=0.02). This was further verified by the use multivariate Cox analysis which included an assessment of the percentage of CD45RO(+) lymphocytes in PCF, age, FIGO status, histology, treatment (CAP or CP) and residual disease (RD) post-surgery. This analysis revealed that two factors had an independent power of prediction: RD ( P=0.02) and the percentage of CD45RO(+) cells in PCF ( P=0.04). Therefore, CD45RO(+) lymphocytes were studied in further detail in a group of 20 patients. This study revealed that PCF CD45RO(+) lymphocytes were characterized by: (1) a higher proportion of cells co-expressing activation markers (HLA-DR, CD28) and higher levels of mRNA for CXC chemokines (IP-10, IL-8) and for IL-10, but with lower levels for IL-2; (2) higher levels of Ki67, bcl-2 and p53 mRNA as compared to those in blood. In conclusion, in the present study it was found that an accumulation of activated CD45RO(+) cells in PCF had a beneficial effect on the survival of patients receiving platinum-based chemotherapy.