Induction of anti-idiotypic humoral and cellular immune responses by a murine monoclonal antibody recognizing the ovarian carcinoma antigen CA125 encapsulated in biodegradable microspheres

The use of biodegradable poly(DL-lactic-co-glycolic acid) microspheres as a cancer vaccine delivery system for induction of anti-idiotypic responses was investigated using a murine monoclonal antibody B43.13 that recognizes the human ovarian cancer antigen CA125. Immunization of mice with mAb B43.13...

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Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 1998-09, Vol.47 (1), p.13-20
Main Authors: JIE MA, SAMUEL, J, KWON, G. S, NOUJAIM, A. A, MADIYALAKAN, R
Format: Article
Language:English
Subjects:
Animals
Antibodies, Anti-Idiotypic - immunology
Antibodies, Monoclonal - blood
Antibodies, Monoclonal - pharmacology
Antibodies, Monoclonal, Murine-Derived
Antibody Formation - immunology
Antineoplastic agents
Biocompatible Materials
Biological and medical sciences
CA-125 Antigen - immunology
Female
Humans
Immunity, Cellular - immunology
Immunotherapy
Lactic Acid
Medical sciences
Mice
Mice, Inbred BALB C
Microspheres
Original
Pharmacology. Drug treatments
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Polymers
T-Lymphocytes - immunology
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Summary:The use of biodegradable poly(DL-lactic-co-glycolic acid) microspheres as a cancer vaccine delivery system for induction of anti-idiotypic responses was investigated using a murine monoclonal antibody B43.13 that recognizes the human ovarian cancer antigen CA125. Immunization of mice with mAb B43.13 encapsulated in poly(DL-lactic-co-glycolic acid) microspheres resulted in enhanced humoral and cellular immune responses compared with mAb B43.13 alone or mAb B43.13 mixed with microspheres. The antibody responses could be further enhanced by the co-encapsulation of mAb B43.13 with monophosphoryl lipid A, a non-toxic adjuvant, in microspheres. Anti-idiotypic humoral responses were shown to result in Ab2 antibodies mimicking the nominal antigen CA125 and Ab3 antibodies recognizing CA125. Further, microsphere delivery of mAb B43.13 also resulted in induction of T cell responses involving T2 cells reactive with mAb B43.13 epitopes and T3 cells recognizing CA125. These results indicate that microsphere delivery of Abl can induce both humoral and cellular anti-idiotypic responses relevant to cancer antigens. This raises the possibility of the use of such formulations for anti-idiotypic induction immunotherapy for cancer.
ISSN:0340-7004
1432-0851
DOI:10.1007/s002620050499