Regulation of proliferation of UM-SCV-1A and UM-SCV-6 vulvar carcinoma cells by cytokines

The biology and pathogenesis of vulvar carcinoma are poorly understood at present. In order to understand this disease better, we have used recently developed squamous cell carcinoma lines of the vulva as models. Two cell lines originating from two individuals (UM-SCV-1A and UM-SCV-6) were cultured...

Full description

Saved in:
Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 1997-01, Vol.43 (6), p.368-374
Main Authors: VIHKO, K. K, SEPPÄNEN, M, HENTTINEN, T, PUNNONEN, J, GRENMAN, S, PUNNONEN, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma, Squamous Cell - pathology
Cell Division - drug effects
Cytokines - pharmacology
DNA Replication - drug effects
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Original
Tumor Cells, Cultured
Tumors
Vulvar Neoplasms - pathology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The biology and pathogenesis of vulvar carcinoma are poorly understood at present. In order to understand this disease better, we have used recently developed squamous cell carcinoma lines of the vulva as models. Two cell lines originating from two individuals (UM-SCV-1A and UM-SCV-6) were cultured in vitro in 10% fetal calf serum. The effects of interleukins 10 and 13, interferons alpha and gamma, granulocyte/macrophage-growth-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF alpha), and transforming growth factor beta (TGFbeta) on the proliferation of the cells was investigated by using radioactively labelled uridine as tracer. In addition, an investigation on the molecular structure of extracted cellular DNA was carried out to investigate whether programmed cell death (apoptosis) would be inducible by any of the factors. In UM-SCV-1A cells, interleukin-10 (IL-10) and interleukin-13 (IL-13) caused an approximately 12-fold decrease in DNA synthesis in cells cultured for 72 h (P
ISSN:0340-7004
1432-0851
DOI:10.1007/s002620050346