Effect of melphalan in vitro on induction of murine suppressor T cells by ConA

The effect of treatment with melphalan in vitro on the activity of spleen cells from BALB/c mice was investigated. Incubation of spleen cells with 1.5-5 micrograms melphalan/1 X 10(7) inhibited subsequent mitogenic stimulation by ConA or PHA and the allogeneic response of BALB/c spleen cells against...

Full description

Saved in:
Bibliographic Details
Published in:Cancer Immunology Immunotherapy 1985-12, Vol.20 (3), p.209-213
Main Authors: OPHIR, R, BEN-EFRAIM, S
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Cells, Cultured
Concanavalin A - pharmacology
General aspects
Immune Tolerance - drug effects
In Vitro Techniques
Lymphocyte Activation - drug effects
Lymphocyte Culture Test, Mixed
Male
Medical sciences
Melphalan - pharmacology
Mice
Mice, Inbred Strains
Original
Pharmacology. Drug treatments
Spleen - cytology
T-Lymphocytes, Regulatory - drug effects
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of treatment with melphalan in vitro on the activity of spleen cells from BALB/c mice was investigated. Incubation of spleen cells with 1.5-5 micrograms melphalan/1 X 10(7) inhibited subsequent mitogenic stimulation by ConA or PHA and the allogeneic response of BALB/c spleen cells against C57B1 target spleen cells. Incubation of spleen cells with ConA led to induction of suppressor T cells which when added to fresh cultures inhibited the allogeneic response. Preincubation of spleen cells with melphalan even at low concentrations (0.15-0.5 micrograms 1 X 10(7) cells) which do not directly affect mitogenic stimulation or allogeneic response partially inhibited the generation of suppressor T cells by ConA. Treatment with melphalan had no effect on already induced suppressor T cells as shown by incubation of spleen cells with melphalan (0.15-5 micrograms/1 X 10(7) cells) after incubation with ConA. Addition of cells treated with melphalan alone (without ConA) to fresh cultures led to an increase in the allogeneic response.
ISSN:0340-7004
1432-0851
DOI:10.1007/BF00205578