Auto-tumor recognition following in vitro induction of MHC antigen expression on solid human tumors: stimulation of lymphocytes and generation of cytotoxicity against the original MHC-antigen-negative tumor cells

Expression of major histocompatibility complex (MHC) class I antigens was induced in eight out of nine freshly prepared tumor cell suspensions by exposure to interferon (IFN gamma) and tumor necrosis factor (TNF alpha) in vitro. The untreated, class-I-antigen-negative, and the treated, antigen-posit...

Full description

Saved in:
Bibliographic Details
Published in:Cancer Immunology Immunotherapy 1989, Vol.28 (1), p.17-21
Main Authors: VANKY, F, STUBER, G, ROTSTEIN, S, KLEIN, E
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Biological and medical sciences
Cytotoxicity, Immunologic
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Antigens Class I - biosynthesis
Histocompatibility Antigens Class I - immunology
Histocompatibility Antigens Class II - biosynthesis
Histocompatibility Antigens Class II - immunology
HLA Antigens - biosynthesis
HLA Antigens - immunology
Humans
Immunobiology
Interferons - pharmacology
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Lymphocytes - immunology
Organs and cells involved in the immune response
Original
Tumor Cells, Cultured - immunology
Tumor Necrosis Factor-alpha - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Expression of major histocompatibility complex (MHC) class I antigens was induced in eight out of nine freshly prepared tumor cell suspensions by exposure to interferon (IFN gamma) and tumor necrosis factor (TNF alpha) in vitro. The untreated, class-I-antigen-negative, and the treated, antigen-positive, cells of three tumors (one breast carcinoma, one plasmocytoma and one ovarian carcinoma) were compared for the capacity to stimulate autologous and allogeneic blood lymphocytes, to generate auto-tumor cytotoxicity and for sensitivity to the lytic effect induced in autologous mixed lymphocyte tumor cell culture (MLTC). The MHC class I-negative cells did not stimulate, while the cells induced for expression of antigens did. On the other hand, when the autologous cytotoxic cells were generated in the MLTC by the class I antigen-positive tumor cells the class I-negative tumor cells were also damaged. Lysis of the class-I-positive tumor cells was abrogated by the W6/32 monoclonal antibody directed against the monomorphic part of the class I molecules.
ISSN:0340-7004
1432-0851
DOI:10.1007/bf00205795