Production of a human monoclonal antibody recognising a determinant on mouse IgG2b from a patient receiving mouse monoclonal antibody for diagnostic imaging

The development of human antibodies recognising mouse immunoglobulins represents an obstacle to effective antibody therapy. This study shows that patients produce modest titres of antibodies (predominantly anti-mouse rather than anti-idiotypic) after a single low-dose injection for immunoscintigraph...

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Bibliographic Details
Published in:Cancer Immunology Immunotherapy 1990-07, Vol.31 (4), p.226-230
Main Authors: DURRANT, L. G, ROBINS, R. A, BALLANTYNE, K. C, AUSTIN, E. B, BALDWIN, R. W
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - isolation & purification
Antibodies, Neoplasm - immunology
Antibody Formation - physiology
Antibody production
Biological and medical sciences
Cell Fusion
Colorectal Neoplasms - diagnostic imaging
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunoglobulin G - immunology
Lymphocytes - physiology
Mice
Original
Radionuclide Imaging
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Summary:The development of human antibodies recognising mouse immunoglobulins represents an obstacle to effective antibody therapy. This study shows that patients produce modest titres of antibodies (predominantly anti-mouse rather than anti-idiotypic) after a single low-dose injection for immunoscintigraphy, suggesting that repeated imaging with the same or a different antibody could be a problem. Fusion of the lymphocytes from a patient who had been imaged twice previously resulted in a monoclonal antibody that specifically binds to an IgG2b isotypic determinant. Anti-IgG2b antibodies predominated in this patient's serum. Production of human monoclonal antibodies from patients given mouse monoclonal antibodies not only allows a finer dissection of the immune repertoire but also provides possible reagents for controlling the human anti-(mouse Ig) response, for selection of class-switch variants of mouse monoclonal antibodies and enhancing tumour imaging.
ISSN:0340-7004
1432-0851
DOI:10.1007/BF01789173