Species-specific TNF induction of thymocyte proliferation

Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF conce...

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Bibliographic Details
Published in:Cancer Immunology Immunotherapy 1988-09, Vol.27 (2), p.103-108
Main Authors: EHRKE, M. J, HO, R. L. X, HORI, K
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Interleukin-1 - pharmacology
Lymphocyte Activation - drug effects
Mice
Mice, Inbred C3H
Organs and cells involved in the immune response
Original
Recombinant Proteins - pharmacology
Species Specificity
T-Lymphocytes - drug effects
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF concentration in a biphasic manner. Thus, 100 to 1,000 units/ml TNF were near optimal while concentrations greater than or equal to 1,000 units/ml caused apparent down regulation. The effect was abrogated by neutralizing antibody to rMu-TNF but not by neutralizing antibody to rMu-interleukin 1 alpha or beta. The rMu-TNF did not induce proliferation of the mature murine T-helper cell line, D10.G4.1, in the presence of mitogen. Taken together the results indicate that TNF, in a strictly species-specific manner, can regulate thymocyte proliferation independently of interleukin 1.
ISSN:0340-7004
1432-0851
DOI:10.1007/BF00200012