Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells

Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haemato...

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Bibliographic Details
Published in:Cancer Immunology Immunotherapy 1992-11, Vol.34 (6), p.407-413
Main Authors: PALISSON, M.-J, ALTEMEYER, A, MOOSBRUGGER, I, WARTER, S, HAUPTMANN, G, BISCHOFF, P
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Binding, Competitive
Biological and medical sciences
Cytotoxicity, Immunologic - immunology
Epitopes - immunology
Host-tumor relations. Immunology. Biological markers
Humans
Karyotyping
Lymphocyte Activation - immunology
Lymphocyte Function-Associated Antigen-1 - immunology
Lymphocytes - immunology
Macromolecular Substances
Mast-Cell Sarcoma - genetics
Mast-Cell Sarcoma - immunology
Mast-Cell Sarcoma - pathology
Medical sciences
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred DBA
Neoplasm Transplantation
Neoplasms, Experimental - genetics
Neoplasms, Experimental - immunology
Neoplasms, Experimental - pathology
Original
Receptors, Fc - immunology
Tumor Cells, Cultured
Tumors
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Summary:Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 alpha chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed.
ISSN:0340-7004
1432-0851
DOI:10.1007/BF01741752