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Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells
Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haemato...
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| Published in: | Cancer Immunology Immunotherapy 1992-11, Vol.34 (6), p.407-413 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c403t-efcc764f367fa36455ddff6b27ea6ec871bc50225255accafda29ba31762771d3 |
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| cites | cdi_FETCH-LOGICAL-c403t-efcc764f367fa36455ddff6b27ea6ec871bc50225255accafda29ba31762771d3 |
| container_end_page | 413 |
| container_issue | 6 |
| container_start_page | 407 |
| container_title | Cancer Immunology Immunotherapy |
| container_volume | 34 |
| creator | PALISSON, M.-J ALTEMEYER, A MOOSBRUGGER, I WARTER, S HAUPTMANN, G BISCHOFF, P |
| description | Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 alpha chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed. |
| doi_str_mv | 10.1007/BF01741752 |
| format | article |
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Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 alpha chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed.</description><identifier>ISSN: 0340-7004</identifier><identifier>EISSN: 1432-0851</identifier><identifier>DOI: 10.1007/BF01741752</identifier><identifier>PMID: 1373342</identifier><identifier>CODEN: CIIMDN</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Binding, Competitive ; Biological and medical sciences ; Cytotoxicity, Immunologic - immunology ; Epitopes - immunology ; Host-tumor relations. Immunology. Biological markers ; Humans ; Karyotyping ; Lymphocyte Activation - immunology ; Lymphocyte Function-Associated Antigen-1 - immunology ; Lymphocytes - immunology ; Macromolecular Substances ; Mast-Cell Sarcoma - genetics ; Mast-Cell Sarcoma - immunology ; Mast-Cell Sarcoma - pathology ; Medical sciences ; Mice ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Neoplasm Transplantation ; Neoplasms, Experimental - genetics ; Neoplasms, Experimental - immunology ; Neoplasms, Experimental - pathology ; Original ; Receptors, Fc - immunology ; Tumor Cells, Cultured ; Tumors</subject><ispartof>Cancer Immunology Immunotherapy, 1992-11, Vol.34 (6), p.407-413</ispartof><rights>1992 INIST-CNRS</rights><rights>Springer-Verlag 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-efcc764f367fa36455ddff6b27ea6ec871bc50225255accafda29ba31762771d3</citedby><cites>FETCH-LOGICAL-c403t-efcc764f367fa36455ddff6b27ea6ec871bc50225255accafda29ba31762771d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11038964/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11038964/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5333463$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1373342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PALISSON, M.-J</creatorcontrib><creatorcontrib>ALTEMEYER, A</creatorcontrib><creatorcontrib>MOOSBRUGGER, I</creatorcontrib><creatorcontrib>WARTER, S</creatorcontrib><creatorcontrib>HAUPTMANN, G</creatorcontrib><creatorcontrib>BISCHOFF, P</creatorcontrib><title>Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells</title><title>Cancer Immunology Immunotherapy</title><addtitle>Cancer Immunol Immunother</addtitle><description>Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 alpha chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Cytotoxicity, Immunologic - immunology</subject><subject>Epitopes - immunology</subject><subject>Host-tumor relations. Immunology. Biological markers</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Function-Associated Antigen-1 - immunology</subject><subject>Lymphocytes - immunology</subject><subject>Macromolecular Substances</subject><subject>Mast-Cell Sarcoma - genetics</subject><subject>Mast-Cell Sarcoma - immunology</subject><subject>Mast-Cell Sarcoma - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Neoplasm Transplantation</subject><subject>Neoplasms, Experimental - genetics</subject><subject>Neoplasms, Experimental - immunology</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Original</subject><subject>Receptors, Fc - immunology</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0340-7004</issn><issn>1432-0851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpVkEtLxDAURoMoOj427oUuRFSo5tl0VjIOjgoDutB1uE0TjbSJJq3gv7fjDI6ucuE7fDf3IHRI8AXBWF5ezzCRnEhBN9CIcEZzXAqyiUaYcZxLjPkO2k3pbRgoHo-30TZhkjFOR2g28Z3LT1_7Fnw2n01ycpa1wQfdBA9NBkNahdqZlFXO19ljSUTW9tF5k3V9G_qYadM0aR9tWWiSOVi9e-h5dvM0vcvnD7f308k81xyzLjdWa1lwywppgRVciLq2tqioNFAYXUpSaYEpFVQI0BpsDXRcASOyoFKSmu2hq2Xve1-1ptbGdxEa9R5dC_FLBXDqf-Ldq3oJn4oQzMpxwYeGk1VDDB-9SZ1qXVrcAN6EPilJy8HdD3i-BHUMKUVjf7cQrBba1Vr7AB_9_dcaXXoe8uNVDklDYyN47dIvJthAFYx9A8NqiPg</recordid><startdate>199211</startdate><enddate>199211</enddate><creator>PALISSON, M.-J</creator><creator>ALTEMEYER, A</creator><creator>MOOSBRUGGER, I</creator><creator>WARTER, S</creator><creator>HAUPTMANN, G</creator><creator>BISCHOFF, P</creator><general>Springer</general><general>Springer-Verlag</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199211</creationdate><title>Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells</title><author>PALISSON, M.-J ; ALTEMEYER, A ; MOOSBRUGGER, I ; WARTER, S ; HAUPTMANN, G ; BISCHOFF, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-efcc764f367fa36455ddff6b27ea6ec871bc50225255accafda29ba31762771d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Cytotoxicity, Immunologic - immunology</topic><topic>Epitopes - immunology</topic><topic>Host-tumor relations. Immunology. Biological markers</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Function-Associated Antigen-1 - immunology</topic><topic>Lymphocytes - immunology</topic><topic>Macromolecular Substances</topic><topic>Mast-Cell Sarcoma - genetics</topic><topic>Mast-Cell Sarcoma - immunology</topic><topic>Mast-Cell Sarcoma - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Neoplasm Transplantation</topic><topic>Neoplasms, Experimental - genetics</topic><topic>Neoplasms, Experimental - immunology</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Original</topic><topic>Receptors, Fc - immunology</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PALISSON, M.-J</creatorcontrib><creatorcontrib>ALTEMEYER, A</creatorcontrib><creatorcontrib>MOOSBRUGGER, I</creatorcontrib><creatorcontrib>WARTER, S</creatorcontrib><creatorcontrib>HAUPTMANN, G</creatorcontrib><creatorcontrib>BISCHOFF, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer Immunology Immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PALISSON, M.-J</au><au>ALTEMEYER, A</au><au>MOOSBRUGGER, I</au><au>WARTER, S</au><au>HAUPTMANN, G</au><au>BISCHOFF, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells</atitle><jtitle>Cancer Immunology Immunotherapy</jtitle><addtitle>Cancer Immunol Immunother</addtitle><date>1992-11</date><risdate>1992</risdate><volume>34</volume><issue>6</issue><spage>407</spage><epage>413</epage><pages>407-413</pages><issn>0340-7004</issn><eissn>1432-0851</eissn><coden>CIIMDN</coden><abstract>Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the alpha and the beta chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 alpha chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>1373342</pmid><doi>10.1007/BF01741752</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0340-7004 |
| ispartof | Cancer Immunology Immunotherapy, 1992-11, Vol.34 (6), p.407-413 |
| issn | 0340-7004 1432-0851 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11038964 |
| source | PubMed Central; Springer LINK Archives |
| subjects | Animals Antibodies, Monoclonal - immunology Antibody Specificity Binding, Competitive Biological and medical sciences Cytotoxicity, Immunologic - immunology Epitopes - immunology Host-tumor relations. Immunology. Biological markers Humans Karyotyping Lymphocyte Activation - immunology Lymphocyte Function-Associated Antigen-1 - immunology Lymphocytes - immunology Macromolecular Substances Mast-Cell Sarcoma - genetics Mast-Cell Sarcoma - immunology Mast-Cell Sarcoma - pathology Medical sciences Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred DBA Neoplasm Transplantation Neoplasms, Experimental - genetics Neoplasms, Experimental - immunology Neoplasms, Experimental - pathology Original Receptors, Fc - immunology Tumor Cells, Cultured Tumors |
| title | Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells |
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