Modern Approaches to de novo Synthesis of Extended DNA Fragments: Assembly of a Wide Repertoire of Sequences

The standardization of DNA fragment assembly methods for many tasks of synthetic biology is crucial. This is necessary for synthesizing a wider repertoire of sequences, as well as for further automation and miniaturization of such reactions. In this work, we proposed conditions for the assembly of D...

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Bibliographic Details
Published in:Actanaturae 2024-01, Vol.16 (1), p.77-85
Main Authors: Semashko, T A, Fisunov, G Y, Tsoy, E A, Kharrasov, D R, Chudinov, I K, Evsyutina, D V, Shevelev, G Y, Govorun, V M
Format: Article
Language:English
Online Access:Get full text
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Summary:The standardization of DNA fragment assembly methods for many tasks of synthetic biology is crucial. This is necessary for synthesizing a wider repertoire of sequences, as well as for further automation and miniaturization of such reactions. In this work, we proposed conditions for the assembly of DNA fragments from chemically synthesized oligonucleotides and we identified the errors occurring in the sequence under these conditions. Additionally, we proposed conditions for further combining synthetic fragments into larger DNA fragments. We showed that the optimized conditions are suitable for the assembly of a wide range of sequences.
ISSN:2075-8251
DOI:10.32607/actanaturae.27362