The RPT2a–MET1 axis regulates TERMINAL FLOWER1 to control inflorescence meristem indeterminacy in Arabidopsis

• Published in: The Plant cell 2024-05, Vol.36 (5), p.1718-1735
• Main Authors: Yao, Wang Jinsong, Wang, Yi Peng, Peng, Jing, Yin, Pei Pei, Gao, Hengbin, Xu, Li, Laux, Thomas, Zhang, Xian Sheng, Su, Ying Hua
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; DNA (Cytosine-5-)-Methyltransferases; DNA Methylation - genetics; Flowers - genetics; Flowers - growth & development; Flowers - metabolism; Gene Expression Regulation, Plant; Inflorescence - genetics; Inflorescence - growth & development; Inflorescence - metabolism; Meristem - genetics; Meristem - growth & development; Meristem - metabolism; Mutation; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 1040-4651; 1532-298X; 1532-298X
• DOI: 10.1093/plcell/koad249

Abstract Plant inflorescence architecture is determined by inflorescence meristem (IM) activity and controlled by genetic mechanisms associated with environmental factors. In Arabidopsis (Arabidopsis thaliana), TERMINAL FLOWER1 (TFL1) is expressed in the IM and is required to maintain indeterminate growth, whereas LEAFY (LFY) is expressed in the floral meristems (FMs) formed at the periphery of the IM and is required to activate determinate floral development. Here, we address how Arabidopsis indeterminate inflorescence growth is determined. We show that the 26S proteasome subunit REGULATORY PARTICLE AAA-ATPASE 2a (RPT2a) is required to maintain the indeterminate inflorescence architecture in Arabidopsis. rpt2a mutants display reduced TFL1 expression levels and ectopic LFY expression in the IM and develop a determinate zigzag-shaped inflorescence. We further found that RPT2a promotes DNA METHYLTRANSFERASE1 degradation, leading to DNA hypomethylation upstream of TFL1 and high TFL1 expression levels in the wild-type IM. Overall, our work reveals that proteolytic input into the epigenetic regulation of TFL1 expression directs inflorescence architecture in Arabidopsis, adding an additional layer to stem cell regulation. The proteasome subunit RPT2a promotes DNA METHYLTRANSFERASE1 degradation, causing DNA hypomethylation upstream of TERMINAL FLOWER1 (TFL1) and high TFL1 levels in the Arabidopsis inflorescence meristem.