The third dimension: new developments in cell culture models for colorectal research

Cellular models are important tools in various research areas related to colorectal biology and associated diseases. Herein, we review the most widely used cell lines and the different techniques to grow them, either as cell monolayer, polarized two-dimensional epithelia on membrane filters, or as t...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2016-11, Vol.73 (21), p.3971-3989
Main Authors: Pereira, Joana F. S., Awatade, Nikhil T., Loureiro, Cláudia A., Matos, Paulo, Amaral, Margarida D., Jordan, Peter
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedical Research
Biomedicine
Cell Biology
Cell culture
Cell Culture Techniques - methods
Cell Line
Colon - cytology
Colorectal cancer
Humans
Life Sciences
Medical research
Models, Biological
Rectum - cytology
Review
Tissue Scaffolds - chemistry
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Summary:Cellular models are important tools in various research areas related to colorectal biology and associated diseases. Herein, we review the most widely used cell lines and the different techniques to grow them, either as cell monolayer, polarized two-dimensional epithelia on membrane filters, or as three-dimensional spheres in scaffold-free or matrix-supported culture conditions. Moreover, recent developments, such as gut-on-chip devices or the ex vivo growth of biopsy-derived organoids, are also discussed. We provide an overview on the potential applications but also on the limitations for each of these techniques, while evaluating their contribution to provide more reliable cellular models for research, diagnostic testing, or pharmacological validation related to colon physiology and pathophysiology.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-016-2258-2