Iron bioleaching and polymers accumulation by an extreme acidophilic bacterium

• Published in: Archives of microbiology 2024-06, Vol.206 (6), p.275, Article 275
• Main Authors: Marchetti, Alessandro, Kupka, Daniel, Senatore, Vittorio Giorgio, Bártová, Zuzana, Branduardi, Paola, Hagarová, Lenka, Hredzák, Slavomír, Lotti, Marina
• Format: Article
• Language: English
• Subjects: Acidiphilium; Acidiphilium - growth & development; Acidiphilium - metabolism; Bacterial leaching; Biochemistry; Biomass; Biomedical and Life Sciences; Biotechnology; Carbon; Carbon sources; Cell Biology; Culture Media - chemistry; Ecology; Galactose; Heavy metals; Hydroxybutyrates - metabolism; Impurities; Iron; Iron - metabolism; Leaching; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Oxidation; Oxidation-Reduction; Oxygenation; Physiology; Polyesters - metabolism; Polyhydroxybutyrate; Polyhydroxybutyrates; Polymers; Polymers - metabolism; Raw materials; Sand; Substrates
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-04005-4

In many European regions, both local metallic and non-metallic raw materials are poorly exploited due to their low quality and the lack of technologies to increase their economic value. In this context, the development of low cost and eco-friendly approaches, such as bioleaching of metal impurities, is crucial. The acidophilic strain Acidiphilium sp. SJH reduces Fe(III) to Fe(II) by coupling the oxidation of an organic substrate to the reduction of Fe(III) and can therefore be applied in the bioleaching of iron impurities from non-metallic raw materials. In this work, the physiology of Acidiphilium sp. SJH and the reduction of iron impurities from quartz sand and its derivatives have been studied during growth on media supplemented with various carbon sources and under different oxygenation conditions, highlighting that cell physiology and iron reduction are tightly coupled. Although the organism is known to be aerobic, maximum bioleaching performance was obtained by cultures cultivated until the exponential phase of growth under oxygen limitation. Among carbon sources, glucose has been shown to support faster biomass growth, while galactose allowed highest bioleaching. Moreover, Acidiphilium sp. SJH cells can synthesise and accumulate Poly-β-hydroxybutyrate (PHB) during the process, a polymer with relevant application in biotechnology. In summary, this work gives an insight into the physiology of Acidiphilium sp. SJH, able to use different carbon sources and to synthesise a technologically relevant polymer (PHB), while removing metals from sand without the need to introduce modifications in the process set up.