Diffusion of d-glucose measured in the cytosol of a single astrocyte

• Published in: Cellular and molecular life sciences : CMLS 2013-04, Vol.70 (8), p.1483-1492
• Main Authors: Kreft, Marko, Lukšič, Miha, Zorec, Tomaž M., Prebil, Mateja, Zorec, Robert
• Format: Article
• Language: English
• Subjects: Animals; Astrocytes; Astrocytes - metabolism; Biochemistry; Biological Transport; Biomedical and Life Sciences; Biomedicine; Biophysics; Brain; Cell Biology; Cells, Cultured; Cellular biology; Cytoplasm; Cytosol - metabolism; Diffusion; Diffusion coefficient; Energy transfer; Fluorescence Resonance Energy Transfer; Glucose; Glucose - metabolism; Kinetics; Life Sciences; Metabolites; Models, Biological; Rats; Research Article; Single-Cell Analysis
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-012-1219-7

Astrocytes interact with neurons and endothelial cells and may mediate exchange of metabolites between capillaries and nerve terminals. In the present study, we investigated intracellular glucose diffusion in purified astrocytes after local glucose uptake. We used a fluorescence resonance energy transfer (FRET)-based nano sensor to monitor the time dependence of the intracellular glucose concentration at specific positions within the cell. We observed a delay in onset and kinetics in regions away from the glucose uptake compared with the region where we locally super-fused astrocytes with the d -glucose-rich solution. We propose a mathematical model of glucose diffusion in astrocytes. The analysis showed that after gradual uptake of glucose, the locally increased intracellular glucose concentration is rapidly spread throughout the cytosol with an apparent diffusion coefficient ( D app ) of (2.38 ± 0.41) × 10 −10  m 2  s −1 (at 22–24 °C). Considering that the diffusion coefficient of d -glucose in water is D  = 6.7 × 10 −10  m 2  s −1 (at 24 °C), D app determined in astrocytes indicates that the cytosolic tortuosity, which hinders glucose molecules, is approximately three times higher than in aqueous solution. We conclude that the value of D app for glucose measured in purified rat astrocytes is consistent with the view that cytosolic diffusion may allow glucose and glucose metabolites to traverse from the endothelial cells at the blood–brain barrier to neurons and neighboring astrocytes.