Technologies of directed protein evolution in vivo

Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2011-04, Vol.68 (7), p.1207-1214
Main Authors: Blagodatski, Artem, Katanaev, Vladimir L
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Cell Biology
Cell culture-based systems
Cellular biology
Directed evolution
Directed Molecular Evolution - methods
Eukaryota - genetics
Eukaryota - metabolism
Evolution
gene conversion
Genetics
Humans
Immunoglobulins
Life Sciences
Mutation
Mutator strain
Prokaryotic Cells - physiology
protein engineering
Protein Engineering - methods
Proteins
Proteins - genetics
Review
Somatic hypermutation
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Summary:Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-010-0610-5