Blocking of melatonin synthesis and MT₁ receptor impairs the activation of Jurkat T cells

Melatonin has been proposed as regulating the immune system by affecting cytokine production in immunocompetent cells, enhancing the production of several T helper (Th)1 cytokines. To further investigate the melatonin's role in IL-2/IL-2R system, we established an inducible T-REx expression sys...

Full description

Saved in:
Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2010-09, Vol.67 (18), p.3163-3172
Main Authors: Lardone, Patricia J, Rubio, Amalia, Cerrillo, Isabel, Gómez-Corvera, Araceli, Carrillo-Vico, Antonio, Sanchez-Hidalgo, Marina, Guerrero, Juan M, Fernandez-Riejos, Patricia, Sanchez-Margalet, Victor, Molinero, Patrocinio
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biosynthesis
Cell Biology
Cellular biology
Cytokines
HIOMT
Hormones
IL-2
Immune system
Life Sciences
Lymphocytes
melatonin
MT
Research Article
T cell receptors
T-REx cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Melatonin has been proposed as regulating the immune system by affecting cytokine production in immunocompetent cells, enhancing the production of several T helper (Th)1 cytokines. To further investigate the melatonin's role in IL-2/IL-2R system, we established an inducible T-REx expression system in Jurkat cells in which the protein levels of HIOMT enzyme or MT₁ receptor were significantly down-regulated upon tetracycline incubation. We found that T-REx Jurkat cells with lower levels of HIOMT activity, and consequently lower content of endogenous melatonin, showed IL-2 production decrease after activation with lectin. Likewise, tetracycline-inducible stable cell line expressing MT₁ antisense produced decreased amounts of IL-2 (mRNA and protein levels) after stimulation. Moreover, in T-Rex-MT₁ cells incubated with tetracycline, a sub-optimal PHA dose failed to induce the early activation marker CD25 on the cell surface. The results shown here support the relevance of endogenous melatonin and its signaling in T cell activation.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-010-0374-y