Blocking of melatonin synthesis and MT₁ receptor impairs the activation of Jurkat T cells

Melatonin has been proposed as regulating the immune system by affecting cytokine production in immunocompetent cells, enhancing the production of several T helper (Th)1 cytokines. To further investigate the melatonin's role in IL-2/IL-2R system, we established an inducible T-REx expression sys...

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Published in:Cellular and molecular life sciences : CMLS 2010-09, Vol.67 (18), p.3163-3172
Main Authors: Lardone, Patricia J, Rubio, Amalia, Cerrillo, Isabel, Gómez-Corvera, Araceli, Carrillo-Vico, Antonio, Sanchez-Hidalgo, Marina, Guerrero, Juan M, Fernandez-Riejos, Patricia, Sanchez-Margalet, Victor, Molinero, Patrocinio
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biosynthesis
Cell Biology
Cellular biology
Cytokines
HIOMT
Hormones
IL-2
Immune system
Life Sciences
Lymphocytes
melatonin
MT
Research Article
T cell receptors
T-REx cells
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cited_by cdi_FETCH-LOGICAL-c395t-1ec1d75118cd8b67cec32a2e0406bef7cc7c8d1b184396466881fd9b4f04353
cites cdi_FETCH-LOGICAL-c395t-1ec1d75118cd8b67cec32a2e0406bef7cc7c8d1b184396466881fd9b4f04353
container_end_page 3172
container_issue 18
container_start_page 3163
container_title Cellular and molecular life sciences : CMLS
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creator Lardone, Patricia J
Rubio, Amalia
Cerrillo, Isabel
Gómez-Corvera, Araceli
Carrillo-Vico, Antonio
Sanchez-Hidalgo, Marina
Guerrero, Juan M
Fernandez-Riejos, Patricia
Sanchez-Margalet, Victor
Molinero, Patrocinio
description Melatonin has been proposed as regulating the immune system by affecting cytokine production in immunocompetent cells, enhancing the production of several T helper (Th)1 cytokines. To further investigate the melatonin's role in IL-2/IL-2R system, we established an inducible T-REx expression system in Jurkat cells in which the protein levels of HIOMT enzyme or MT₁ receptor were significantly down-regulated upon tetracycline incubation. We found that T-REx Jurkat cells with lower levels of HIOMT activity, and consequently lower content of endogenous melatonin, showed IL-2 production decrease after activation with lectin. Likewise, tetracycline-inducible stable cell line expressing MT₁ antisense produced decreased amounts of IL-2 (mRNA and protein levels) after stimulation. Moreover, in T-Rex-MT₁ cells incubated with tetracycline, a sub-optimal PHA dose failed to induce the early activation marker CD25 on the cell surface. The results shown here support the relevance of endogenous melatonin and its signaling in T cell activation.
doi_str_mv 10.1007/s00018-010-0374-y
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subjects Biochemistry
Biomedical and Life Sciences
Biomedicine
Biosynthesis
Cell Biology
Cellular biology
Cytokines
HIOMT
Hormones
IL-2
Immune system
Life Sciences
Lymphocytes
melatonin
MT
Research Article
T cell receptors
T-REx cells
title Blocking of melatonin synthesis and MT₁ receptor impairs the activation of Jurkat T cells
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