(-)-Epigallocatechin-3-gallate interferes with mast cell adhesiveness, migration and its potential to recruit monocytes

• Published in: Cellular and molecular life sciences : CMLS 2007-10, Vol.64 (19-20), p.2690-2701
• Main Authors: Melgarejo, E, Medina, M. A, Sánchez-Jiménez, F, Botana, L. M, Domínguez, M, Escribano, L, Orfao, A, Urdiales, J. L
• Format: Article
• Language: English
• Subjects: Adhesion; Antineoplastic Agents - pharmacology; Catechin - analogs & derivatives; Catechin - pharmacology; Cell adhesion & migration; Cell Adhesion - drug effects; Cell Line; Cell Movement - drug effects; chemokines; Cytokines; Gene expression; Gene Expression Profiling; Gene Expression Regulation - drug effects; Green tea; Humans; Immune system; integrin α5; integrin β3; Integrins - metabolism; mast cells; Mast Cells - cytology; monocyte chemoattractant protein-1; Monocytes - cytology; Neovascularization, Physiologic - drug effects
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-007-7331-4

Mast cells are multipotent effector cells of the immune system. They are able to induce and enhance angiogenesis via multiple pathways. (-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea and a putative chemopreventive agent, was reported to inhibit tumor invasion and angiogenesis, processes that are essential for tumor growth and metastasis. Using the human mast cell line HMC-1 and commercial cDNA macroarrays, we evaluated the effect of EGCG on the expression of angiogenesis-related genes. Our data show that among other effects, EGCG treatment reduces expression of two integrins (α5 and β3) and a chemokine (MCP1), resulting in a lower adhesion of mast cells associated with a decreased potential to produce signals eliciting monocyte recruitment. These effects on gene expression levels are functionally validated by showing inhibitory effects in adhesion, aggregation, migration and recruitment assays.