Identification of factor XIII-A as a marker of alternative macrophage activation

Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was des...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2005-09, Vol.62 (18), p.2132-2139
Main Authors: Töröcsik, D, Bárdos, H, Nagy, L, Adány, R
Format: Article
Language:English
Subjects:
Biomarkers - analysis
Biomarkers - metabolism
Cell Differentiation - genetics
Coagulation
Dendritic Cells - immunology
Factor XIIIa - analysis
Factor XIIIa - genetics
Factor XIIIa - metabolism
Gene Expression
Gene Expression Regulation
Genetic markers
Humans
Interferon-gamma - pharmacology
Interleukin-4 - pharmacology
Macrophage Activation - genetics
Macrophages - immunology
Monocytes - drug effects
Monocytes - microbiology
Mycobacterium bovis
Protein Biosynthesis
Proteins
RNA, Messenger - analysis
RNA, Messenger - metabolism
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Summary:Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was designed to monitor the changes in FXIII-A gene expression and protein production in long-term culture of human monocytes during their differentiation into macrophages in the presence of activating agents (interleukin-4, interferon-gamma, Mycobacterium bovis BCG) inducing classical and alternative activation pathways. By using quantitative RT-PCR and fluorescent image analysis at the single-cell level we demonstrated that the expression of FXIII-A both at the mRNA as well as at the protein level is inversely regulated during the two activation programmes. Here we conclude that FXIII-A expression is an intracellular marker for alternatively activated macrophages, while its absence in monocyte-derived macrophages indicates their classically activated state.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-005-5242-9