Crystallization of RNA

Even as the number of RNA structures determined and under study multiplies, the critical step in X-ray diffraction analysis, growth of single well-ordered crystals, remains at the boundary between art and science. Recent advances in methods of RNA synthesis, purification, and characterization, as we...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2001-02, Vol.58 (2), p.234-243
Main Authors: Holbrook, S R, Holbrook, E L, Walukiewicz, H E
Format: Article
Language:English
Subjects:
Crystallization
Crystallography
Crystallography, X-Ray
Crystals
Molecular biology
Ribonucleic acid
RNA
RNA - chemical synthesis
RNA - chemistry
RNA - isolation & purification
Structural analysis
X-ray diffraction
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Description
Summary:Even as the number of RNA structures determined and under study multiplies, the critical step in X-ray diffraction analysis, growth of single well-ordered crystals, remains at the boundary between art and science. Recent advances in methods of RNA synthesis, purification, and characterization, as well as empirical and technical improvements in crystallization techniques, the development of cryo-crystallography, and the wider availability of bright, tunable, X-rays from synchrotron sources are improving the chances of obtaining RNA crystals suitable for X-ray structural analysis. In this review, we summarize the current status of the design, preparation, purification, and analysis of RNA for crystallization and describe the latest approaches to obtaining diffraction-quality crystals.
ISSN:1420-682X
1420-9071
DOI:10.1007/PL00000851