Hyperthermia-induced apoptosis and the inhibition of DNA laddering by zinc supplementation and withdrawal of calcium and magnesium in suspension culture of tobacco cells

In the present paper we report examination of stereotypic hallmarks of apoptosis in heat-treated tobacco cells. Hyperthermia (44 °C, 4 h) caused apoptosis in 53.6% of cells when assayed 24 h after heat treatment. The induction of apoptosis by heat treatment was confirmed by flow cytometric assay. Cy...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 1999-02, Vol.55 (2), p.303-309
Main Authors: Chen, H. (Tsinghua University, Beijing China.), Yan, C, Jiang, X, Dai, Y.R
Format: Article
Language:English
Subjects:
ADN
APOPTOSE
APOPTOSIS
CALCIO
CALCIUM
CATION
CATIONES
CATIONS
CELL CULTURE
CELL SUSPENSIONS
Cellular biology
CHOC
CINC
CITOLOGIA
CULTIVO DE CELULAS
CULTURE DE CELLULE
CULTURE MEDIA
CYTOLOGIE
CYTOLOGY
CYTOTOXICITY
Deoxyribonucleic acid
DNA
DNA CLEAVAGE
DNA FRAGMENTATION
DNA MODIFICATION
DNA, Plant - analysis
DNA, Plant - genetics
ESTRES TERMICO
HEAT SHOCK
HEAT STRESS
Heat treating
HEAT TREATMENT
Heat-Shock Response
In Situ Nick-End Labeling
MAGNESIO
MAGNESIUM
MEDIO DE CULTIVO
MILIEU DE CULTURE
Nicotiana - cytology
Nicotiana - genetics
Nicotiana - physiology
NICOTIANA TABACUM
SHOCK
STRESS THERMIQUE
THERMAL STRESS
Tobacco
TOXICIDAD
TOXICITE
TOXICITY
TRAITEMENT THERMIQUE
TRATAMIENTO TERMICO
ZINC
Zinc - metabolism
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Summary:In the present paper we report examination of stereotypic hallmarks of apoptosis in heat-treated tobacco cells. Hyperthermia (44 °C, 4 h) caused apoptosis in 53.6% of cells when assayed 24 h after heat treatment. The induction of apoptosis by heat treatment was confirmed by flow cytometric assay. Cytological observations revealed condensation of the cytoplasm and nucleus, as well as nuclear collapse. DNA ladders were observed in DNA extracted from heat-treated cells, whereas DNA from control cells remained undegraded. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay revealed that 51.8% of the heat-treated cells (44 °C, 4 h) show positive reaction after a 24-h recovery. When cells were cultured in a medium supplemented with 0.4-5.0 mM ZnSO4, internucleosomal DNA fragmentation induced by heat shock was completely negated. Strikingly, when cells were cultured in Ca(2+) and/or Mg(2+) free medium for 44 h followed by heat treatment, DNA laddering was not observed. The results suggest hyperthermia-induced apoptosis and a correlation between the regulation of endonucleases and heat shock signal in apoptotic tobacco cells.
ISSN:1420-682X
1420-9071
DOI:10.1007/s000180050292