Immunometabolic crosstalk in Aedes fluviatilis and Wolbachia pipientis symbiosis

Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host's physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes...

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Bibliographic Details
Published in:The Journal of biological chemistry 2024-06, Vol.300 (6), p.107272, Article 107272
Main Authors: Nascimento da Silva, Jhenifer, Conceição, Christiano Calixto, Ramos de Brito, Gisely Cristina, Renato de Oliveira Daumas Filho, Carlos, Walter Nuno, Ana Beatriz, Talyuli, Octavio A.C., Arcanjo, Angélica, de Oliveira, Pedro L., Moreira, Luciano Andrade, Vaz Jr, Itabajara da Silva, Logullo, Carlos
Format: Article
Language:English
Subjects:
Aedes - immunology
Aedes - metabolism
Aedes - microbiology
Animals
Glycogen - metabolism
Glycogen Synthase Kinase 3 beta - genetics
Glycogen Synthase Kinase 3 beta - metabolism
GSK3β
immunometabolism
Insect Proteins - genetics
Insect Proteins - metabolism
Relish2
Symbiosis
Wolbachia
Wolbachia - metabolism
Wolbachia - physiology
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Summary:Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host's physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes fluviatilis (Ae. fluviatilis). Glycogen synthesis is controlled by the enzyme GSK3, which is also involved in immune responses in both vertebrate and invertebrate organisms. Here we investigated the mechanisms behind immune changes mediated by glycogen synthase kinase β (GSK3β) in the symbiosis between Ae. fluviatilis and W. pipientis using a GSK3β inhibitor or RNAi-mediated gene silencing. GSK3β inhibition or knockdown increased glycogen content and Wolbachia population, together with a reduction in Relish2 and gambicin transcripts. Furthermore, knockdown of Relish2 or Caspar revealed that the immunodeficiency pathway acts to control Wolbachia numbers in the host. In conclusion, we describe for the first time the involvement of GSK3β in Ae. fluviatilis immune response, acting to control the Wolbachia endosymbiotic population.
ISSN:0021-9258
1083-351X
1083-351X
DOI:10.1016/j.jbc.2024.107272