DMRT‐1 expression during NEC8 human embryonic carcinoma cell differentiation

To elucidate the relationship between dsx and mab‐3 related transcription factor 1 (Dmrt‐1) and differentiation, alteration in mRNA levels during differentiation of NEC8 human embryonic carcinoma cells was investigated. After stimulation with 50 nM phorbol 12‐myristate 13‐acetate (PMA), the cells di...

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Published in:Cancer science 2006-04, Vol.97 (4), p.277-282
Main Authors: Koji, Hitoshi, Yamada, Akira, Nagasawa, Toshihiko, Gamou, Shinobu
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Carcinogens - pharmacology
Carcinoma, Embryonal - metabolism
Carcinoma, Embryonal - pathology
Carrier Proteins - metabolism
Cell Differentiation
Cells, Cultured
Humans
Male
Mesoderm - metabolism
Mesoderm - pathology
Mitogen-Activated Protein Kinase 1 - metabolism
Original
Protein Kinase C - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - pharmacology
Signal Transduction
Testis - metabolism
Testis - pathology
Tetradecanoylphorbol Acetate - pharmacology
Transcription Factors - antagonists & inhibitors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:To elucidate the relationship between dsx and mab‐3 related transcription factor 1 (Dmrt‐1) and differentiation, alteration in mRNA levels during differentiation of NEC8 human embryonic carcinoma cells was investigated. After stimulation with 50 nM phorbol 12‐myristate 13‐acetate (PMA), the cells differentiated into cells with mesenchymal characteristics and upregulated Dmrt‐1 mRNA, possibly through the protein kinase C/mitogen‐activated protein kinase/activated protein‐1 signaling pathway. Conversely, knockdown of Dmrt‐1 by small interfering RNA resulted in cell morphology that was different from that after PMA treatment. These results indicated that Dmrt‐1 expression was apparently associated with the differentiation of NEC8, and this cell line may be a helpful in vitro tool to clarify the role of Dmrt‐1 in the differentiation process. (Cancer Sci 2006; 97: 277 –282)
ISSN:1347-9032
1349-7006
DOI:10.1111/j.1349-7006.2006.00178.x