Condensin mutations and abnormal chromosomal structures in pyothorax‐associated lymphoma

Transfer of genetic information during mitosis is accurately conducted by proper condensation and segregation of chromosomes, for which condensins play a central role. Both condensin I and II have common structural maintenance of chromosomes subunits, named hCAP‐C and hCAP‐E. Pyothorax‐associated ly...

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Published in:Cancer science 2007-07, Vol.98 (7), p.1041-1047
Main Authors: Ham, Maria Francisca, Takakuwa, Tetsuya, Rahadiani, Nur, Tresnasari, Kristianti, Nakajima, Hiroo, Aozasa, Katsuyuki
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Biological and medical sciences
Cell Line, Tumor
Chromosome Aberrations
DNA-Binding Proteins - genetics
Empyema, Pleural - genetics
Empyema, Pleural - pathology
HeLa Cells
Humans
Karyotyping
Leukemia - genetics
Leukemia - pathology
Lymphoma - genetics
Lymphoma - pathology
Medical sciences
Metaphase
Multiprotein Complexes - genetics
Mutation
Original
Reverse Transcriptase Polymerase Chain Reaction
RNA, Small Interfering - genetics
Tumors
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Summary:Transfer of genetic information during mitosis is accurately conducted by proper condensation and segregation of chromosomes, for which condensins play a central role. Both condensin I and II have common structural maintenance of chromosomes subunits, named hCAP‐C and hCAP‐E. Pyothorax‐associated lymphoma (PAL) is a non‐Hodgkin's lymphoma developing in the pleural cavity of patients with long‐standing pyothorax. Mutations of hCAP‐C and hCAP‐E were investigated in 24 leukemia–lymphoma cell lines including eight PAL cell lines, and their influences in chromosome morphology were evaluated. Heterozygous point mutations within hCAP‐C were found in two PAL cell lines and corresponding tumor samples (OPL‐3 and OPL‐7). Deletion of exon 24 within hCAP‐E and a point mutation at the donor splice site of intron 24 were detected in OPL‐5 and original tumor samples. OPL‐5 showed an extensive reduction in expression of not only hCAP‐E but also hCAP‐C proteins. OPL‐5 occasionally showed the chromosome bridge in anaphase and telophase, indicating that segregation is not accurate. OPL‐7 showed reduced hCAP‐C protein expression, abnormality in chromosome length and width, and abnormal aggregates of hCAP‐C protein. These findings indicated that condensin gene alteration might play a role in genome instability, which accelerates the accumulation of other gene alterations in PAL. (Cancer Sci 2007; 98: 1041–1047)
ISSN:1347-9032
1349-7006
DOI:10.1111/j.1349-7006.2007.00500.x