Detection of p16 promoter hypermethylation in serum of gastric cancer patients

For the early detection of tumor‐related aberrant DNA in the serum of patients, we examined promoter hypermethylation of the p16 gene using methylation‐specific PCR (MSP) in paired tumor and serum samples of 60 gastric cancer patients. Aberrant p16 methylation was found in 23 of 60 (38%) primary gas...

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Bibliographic Details
Published in:Cancer science 2003-05, Vol.94 (5), p.418-420
Main Authors: Kanyama, Yasuaki, Hibi, Kenji, Nakayama, Hiroshi, Kodera, Yasuhiro, Ito, Katsuki, Akiyama, Seiji, Nakao, Akimasa
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Case-Control Studies
Cyclin-Dependent Kinase Inhibitor p16 - genetics
Deoxyribonucleic acid
DNA
DNA Methylation
DNA, Neoplasm - blood
Female
Gastric cancer
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms - blood
Liver Neoplasms - secondary
Lymphatic Metastasis
Male
Medical sciences
Middle Aged
Neoplasm Invasiveness - pathology
Neoplasm Staging
p16 Protein
Peritoneal Neoplasms - blood
Peritoneal Neoplasms - secondary
Promoter Regions, Genetic
Stomach Neoplasms - blood
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:For the early detection of tumor‐related aberrant DNA in the serum of patients, we examined promoter hypermethylation of the p16 gene using methylation‐specific PCR (MSP) in paired tumor and serum samples of 60 gastric cancer patients. Aberrant p16 methylation was found in 23 of 60 (38%) primary gastric cancers, but in none of the corresponding gastric mucosae. Of these 23 patients, 6 (26%) exhibited the same alteration in their serum DNA. As a control, we screened for aberrant methylation in the serum DNA of 37 patients with gastric cancers whose corresponding tumor DNA had no methylation in the p16 promoter. We also screened for methylation in the serum DNA of 16 non‐cancer individuals. No methylation was found in serum DNA of these control groups. Our results suggest that p16 methylation would be a good marker for the detection of tumor DNA in the serum of primary gastric cancer patients. (Cancer Sci 2003; 94: 418–420)
ISSN:1347-9032
1349-7006
DOI:10.1111/j.1349-7006.2003.tb01457.x