PbARID-associated chromatin remodeling events are essential for gametocyte development in Plasmodium

• Published in: Nucleic acids research 2024-03, Vol.52 (10), p.5624-5642
• Main Authors: Nishi, Tsubasa, Kaneko, Izumi, Iwanaga, Shiroh, Yuda, Masao
• Format: Article
• Language: English
• Subjects: Animals; Chromatin Assembly and Disassembly - genetics; Female; Gene regulation, Chromatin and Epigenetics; Male; Mice; Plasmodium berghei - genetics; Plasmodium berghei - growth & development; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gkae207

Gametocyte development of the Plasmodium parasite is a key step for transmission of the parasite. Male and female gametocytes are produced from a subpopulation of asexual blood-stage parasites, but the mechanisms that regulate the differentiation of sexual stages are still under investigation. In this study, we investigated the role of PbARID, a putative subunit of a SWI/SNF chromatin remodeling complex, in transcriptional regulation during the gametocyte development of P. berghei. PbARID expression starts in early gametocytes before the manifestation of male and female-specific features, and disruption of its gene results in the complete loss of gametocytes with detectable male features and the production of abnormal female gametocytes. ChIP-seq analysis of PbARID showed that it forms a complex with gSNF2, an ATPase subunit of the SWI/SNF chromatin remodeling complex, associating with the male cis-regulatory element, TGTCT. Further ChIP-seq of PbARID in gsnf2-knockout parasites revealed an association of PbARID with another cis-regulatory element, TGCACA. RIME and DNA-binding assays suggested that HDP1 is the transcription factor that recruits PbARID to the TGCACA motif. Our results indicated that PbARID could function in two chromatin remodeling events and paly essential roles in both male and female gametocyte development.