Evidence of interactions among apoptosis, cell proliferation, and dedifferentiation in the rudiment during whole-organ intestinal regeneration in the sea cucumber

• Published in: Developmental biology 2024-01, Vol.505, p.99-109
• Main Authors: Reyes-Rivera, Josean, Grillo-Alvarado, Valentina, Soriano-López, Ashley E., García-Arrarás, José E.
• Format: Article
• Language: English
• Subjects: Animals; Anlage formation; Aphidicolin; Apoptosis; Apoptosis-induced proliferation; Cell Dedifferentiation; Cell Proliferation; Compensatory mechanism; digestive tract; Holothuria; Intestinal regeneration; Intestines; mesentery; mitomycin; muscles; Regeneration; Sea cucumber; Sea Cucumbers - physiology
• ISSN: 0012-1606; 1095-564X; 1095-564X
• DOI: 10.1016/j.ydbio.2023.11.001

Sea cucumbers have an extraordinary regenerative capability. Under stressful conditions, Holothuria glaberrima can eviscerate their internal organs, including the digestive tract. From the mesentery, a rudiment grows and gives rise to a new intestine within a few weeks. In the last decades, the cellular events that occur during intestinal regeneration have been characterized, including apoptosis, cell proliferation, and muscle cell dedifferentiation. Nevertheless, their contribution to the formation and early growth of the rudiment is still unknown. Furthermore, these cellular events' relationship and potential interdependence remain a mystery. Using modulators to inhibit apoptosis and cell proliferation, we tested whether rudiment growth or other regenerative cellular events like muscle cell dedifferentiation were affected. We found that inhibition of apoptosis by zVAD and cell proliferation by aphidicolin and mitomycin did not affect the overall size of the rudiment seven days post-evisceration (7-dpe). Interestingly, animals treated with aphidicolin showed higher levels of muscle cell dedifferentiation in the distal mesentery, which could act as a compensatory mechanism. On the other hand, inhibition of apoptosis led to a decrease in cell proliferation in the rudiment and a delay in the spatiotemporal progression of muscle cell dedifferentiation throughout the rudiment-mesentery structure. Our findings suggest that neither apoptosis nor cell proliferation significantly contributes to early rudiment growth during intestinal regeneration in the sea cucumber. Nevertheless, apoptosis may play an essential role in modulating cell proliferation in the rudiment (a process known as apoptosis-induced proliferation) and the timing for the progression of muscle cell dedifferentiation. These findings provide new insights into the role and relationship of cellular events during intestinal regeneration in an emerging regeneration model. [Display omitted] •Neither apoptosis nor cell proliferation play a critical role in rudiment formation.•Apoptosis-induced proliferation occurs during holothurian intestinal regeneration.•Apoptosis regulates the spatiotemporal progression of muscle cell dedifferentiation.•Muscle cell dedifferentiation acts as a compensatory mechanism during regeneration.