Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress

Chitin degradation products, especially chitosan oligosaccharides (COSs), are highly valued in various industrial fields, such as food, medicine, cosmetics and agriculture, for their rich resources and high cost-effectiveness. However, little is known about the impact of acetylation on COS cellular...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2024-08, Vol.28 (2), Article 310
Main Authors: Li, Qiongyu, Shi, Wan-Rong, Huang, Yun-Lin
Format: Article
Language:English
Subjects:
Alzheimer's disease
Analysis
Antioxidants
Apoptosis
Atherosclerosis
Biological activity
Biotechnology
Care and treatment
Cell cycle
Chitin
Defense mechanisms
DNA damage
Flow cytometry
Health aspects
Inflammation
Mass spectrometry
Molecular weight
Nitric oxide
Oxidative stress
Penicillin
Polymerization
Scientific imaging
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Summary:Chitin degradation products, especially chitosan oligosaccharides (COSs), are highly valued in various industrial fields, such as food, medicine, cosmetics and agriculture, for their rich resources and high cost-effectiveness. However, little is known about the impact of acetylation on COS cellular bioactivity. The present study aimed to compare the differential effects of COS and highly N-acetylated COS (NACOS), known as chitin oligosaccharide, on [H.sub.2][O.sub.2]-induced cell stress. MTT assay showed that pretreatment with NACOS and COS markedly inhibited [H.sub.2][O.sub.2]-induced RAW264.7 cell death in a concentration-dependent manner. Flow cytometry indicated that NACOS and COS exerted an anti-apoptosis effect on [H.sub.2][O.sub.2]-induced oxidative damage in RAW264.7 cells. NACOS and COS treatment ameliorated [H.sub.2][O.sub.2]-induced RAW264.7 cell cycle arrest. Western blotting revealed that the anti-oxidation effects of NACOS and COS were mediated by suppressing expression of proteins involved in [H.sub.2][O.sub.2]-induced apoptosis, including Bax, Bcl-2 and cleaved PARP. Furthermore, the antagonist effects of NACOS were greater than those of COS, suggesting that acetylation was essential for the protective effects of COS. Key words: N-acetylated chitosan oligosaccharide, apoptosis
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2024.12600