H2BFWT Variations in Sperm DNA and Its Correlation to Pregnancy

Abnormalities in sperm nuclei and chromatin can interfere with normal fertilization, embryonic development, implantation, and pregnancy. We aimed to study the impact of gene variants in sperm DNA on ICSI outcomes in couples undergoing ART treatment. One hundred and nineteen partners were divided int...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-06, Vol.25 (11), p.6048
Main Authors: Amor, Houda, Juhasz-Böss, Ingolf, Bibi, Riffat, Hammadeh, Mohamad Eid, Jankowski, Peter Michael
Format: Article
Language:English
Subjects:
Adult
Alleles
Chromatin
Couples
DNA
DNA - genetics
DNA methylation
Embryonic development
Epigenetic inheritance
Epigenetics
Female
Fertility
Gene expression
Genes
Genetic transcription
Genomes
Histones - genetics
Humans
Infertility
Male
Polymorphism
Polymorphism, Single Nucleotide
Pregnancy
Pregnant women
Proteins
Quality control
Reproductive technologies
Sperm
Sperm Injections, Intracytoplasmic - methods
Spermatogenesis
Spermatozoa
Spermatozoa - metabolism
X chromosomes
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Summary:Abnormalities in sperm nuclei and chromatin can interfere with normal fertilization, embryonic development, implantation, and pregnancy. We aimed to study the impact of gene variants in sperm DNA on ICSI outcomes in couples undergoing ART treatment. One hundred and nineteen partners were divided into pregnant (G1) and non-pregnant (G2) groups. After semen analysis, complete DNA was extracted from purified sperm samples. The sequence of the gene was amplified by PCR and then subjected to Sanger sequencing. The results showed that there are three mutations in this gene: rs7885967, rs553509, and rs578953. Significant differences were shown in the distribution of alternative and reference alleles between G1 and G2 ( = 0.0004 and = 0.0020, respectively) for rs553509 and rs578953. However, there was no association between these SNPs and the studied parameters. This study is the first to shed light on the connection between gene variants in sperm DNA and pregnancy after ICSI therapy. This is a pilot study, so further investigations about these gene variants at the transcriptional and translational levels will help to determine its functional consequences and to clarify the mechanism of how pregnancy can be affected by sperm DNA.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25116048