BIOM-12. LIQUID BIOPSIES IN CNS TUMORS: A COMBINED APPROACH TO PROFILING CSF

• Published in: Neuro-oncology (Charlottesville, Va.) Va.), 2024-06, Vol.26 (Supplement_4), p.0-0
• Main Authors: Nobre, Liana, Nakano, Yoshiko, Burns, Ian, Johnson, Monique, Sheth, Javal, Rana, Mansuba, Yuditskiy, Richard, Negm, Logine, Zapotocky, Michal, Stucklin, Ana, Erker, Craig, Cacciotti, Chantel, Fleming, Adam, Cheng, Sylvia, Huang, Annie, Bondoc, Andrew, Fat, MaryJane Lim, Das, Anirban, Magimairajan, Vanan, Bennet, Julie, Siddaway, Robert, Tabori, Uri, Hawkins, Cynthia
• Format: Article
• Language: English
• Subjects: Final category: Biological Correlates, Biomarkers
• ISSN: 1522-8517; 1523-5866
• DOI: 10.1093/neuonc/noae064.031

In precision medicine, molecular profiling of pediatric tumors is crucial for diagnosis, prognosis, and therapeutic decision-making. Traditional biopsy methods, often invasive, have inherent risks and may not capture a tumor’s heterogeneity. Liquid biopsy, however, offers a minimally invasive and comprehensive alternative. We demonstrate its utility in diagnosing and monitoring brain tumors using cell-free DNA (cfDNA) from cerebrospinal fluid (CSF), where conventional cfDNA plasma analysis has shown low sensitivity. Through digital droplet PCR, we analyzed 23 plasma samples from patients with BRAFV600E or H3K27M gliomas, detecting H3K27M in a single case. Conversely, CSF samples revealed 8 positives out of 9 for these mutations, underscoring CSF’s diagnostic value. We advanced our methodology by developing a 21-gene hybrid-capture panel paired with low-pass whole-genome sequencing to pinpoint copy number variations. In our reference sample set, the panel achieved a detection limit with variant allele frequencies as low as 0.5%. With 10ng of cfDNA, we reached 83% sensitivity and 100% specificity, which increased to 100% sensitivity with a 30ng input. Using this assay, we profiled 141 CSF samples from 119 patients, achieving 70% positivity rate in active disease cases in a validation cohort—ranging from 50% in low-grade gliomas to 84% in high-grade gliomas and 75% in medulloblastomas. Intriguingly, of 14 queried tumor samples lacking biopsy confirmation, half showed positive ctDNA results using the panel and/or low-pass whole-genome sequencing. This approach allowed for differential diagnoses in suspected medulloblastoma relapses, tailored therapeutic interventions based on CSF profiles, and effective treatment response monitoring. In summary, our integrated liquid biopsy strategy showcases a powerful, clinically applicable tool that significantly enhances patient care in pediatric neuro-oncology.