Evaluation of the antibacterial and cytotoxic properties of TotalFill and NeoSEALER flo bioceramic sealers

• Published in: Journal of conservative dentistry and endodontics 2024-05, Vol.27 (5), p.491-497
• Main Authors: Sebastian, Sabatiny, El-Sayed, Walid, Adtani, Pooja, Zaarour, Rania Faouzi, Nandakumar, Aswathy, Elemam, Ranya F, Eid, Bassem, Elsewify, Tarek
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2950-4716; 2950-4708; 2950-4708
• DOI: 10.4103/JCDE.JCDE_103_24

Evaluation of the antibacterial and cytotoxic properties of TotalFill and NeoSEALER Flo bioceramic sealers compared to AH Plus resin sealer. Modified direct contact test was used on three sets of sealers: Freshly mixed sealers, sealers that were 1-day old, and sealers that were 7-day old. After 24 h of incubation, the colony-forming units were digitally counted using Promega Colony Counter after 30 and 60 min of exposure to . For cytotoxic effect evaluation, 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay was performed at three different time points: 24 h, 48 h, and 120 h after adding the sealer eluates to human gingival fibroblasts, to assess cell viability. Data were analyzed using mixed model analysis of variance followed by test. TotalFill bioceramic sealer showed the highest bacterial reduction against throughout all intervals. AH Plus showed great antibacterial activity initially which reduced drastically after 7 days. All the sealers showed a reduction in their antibacterial activity with time. TotalFill and NeoSEALER Flo showed very high cell viability in contrast to AH Plus. TotalFill and NeoSEALER Flo demonstrate superior antimicrobial properties against which reduces with time. TotalFill and NeoSEALER Flo demonstrate acceptable biocompatibility against human gingival fibroblasts, which decreased over time.