Antioxidant 1 copper chaperone gene expression and copper levels in dog osteosarcoma patients

Twenty‐four dogs with OS underwent limb amputation. Serum, OS tumour, and normal bone were harvested at time of surgery. RNA was extracted and gene expression was performed using quantitative polymerase chain reaction (qPCR). Tissue and blood copper concentrations were also determined with spectroph...

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Bibliographic Details
Published in:Veterinary & comparative oncology 2023-09, Vol.21 (3), p.559-564
Main Authors: Rivera, Pedro L., Li, William T., Bhogal, Sumail, Mandell, Jonathan B., Belayneh, Rebekah, Hankins, Margaret L., Payne, John T., Watters, Rebecca J., Weiss, Kurt R.
Format: Article
Language:English
Subjects:
ALDH
Animals
Antioxidants
Bone Neoplasms - genetics
Bone Neoplasms - veterinary
canine
Copper
Copper Transport Proteins - genetics
Dog Diseases - genetics
Dog Diseases - metabolism
Dogs
Gene Expression
Humans
Mice
Molecular Chaperones - genetics
Molecular Chaperones - metabolism
osteosarcoma
Osteosarcoma - genetics
Osteosarcoma - metabolism
Osteosarcoma - veterinary
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Summary:Twenty‐four dogs with OS underwent limb amputation. Serum, OS tumour, and normal bone were harvested at time of surgery. RNA was extracted and gene expression was performed using quantitative polymerase chain reaction (qPCR). Tissue and blood copper concentrations were also determined with spectrophotometry. Compared to bone, tumour samples had significantly higher expressions of antioxidant 1 copper chaperone (ATOX1, p = .0003). OS tumour copper levels were significantly higher than that of serum (p 
ISSN:1476-5810
1476-5829
1476-5829
DOI:10.1111/vco.12903