Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol

• Published in: Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.419, Article 419
• Main Authors: Magalhães, Carla P., Alves, Joana I., Duber, Anna, Oleskowicz-Popiel, Piotr, Stams, Alfons J. M., Cavaleiro, Ana J.
• Format: Article
• Language: English
• Subjects: Acetates - metabolism; Anaerobic conditions; Anaerobic microorganisms; Anaerobiosis; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biodiesel fuels; Biofuels; Biomedical and Life Sciences; Biotechnology; Environmental Biotechnology; Environmental conditions; Fermentation; Glycerol; Glycerol - metabolism; Industrial applications; Inoculum; Life Sciences; Metabolism; Methane; Methane - metabolism; Microbial Genetics and Genomics; Microbiology; Microbiota; Microorganisms; Phylogeny; Propionates - metabolism; Propionic acid; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sewage - microbiology; Sludge; Sulfate reduction; Sulfates; Sulfates - metabolism; Versatility
• ISSN: 0175-7598; 1432-0614; 1432-0614
• DOI: 10.1007/s00253-024-13248-6

Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from the same inoculum (anaerobic granular sludge), with high microbial specialization, within three different phyla ( Thermodesulfobacteriota , Euryarchaeota and Pseudomonadota ). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to Solidesulfovibrio alcoholivorans (99.8% 16S rRNA gene identity), in syntrophic relationship with Methanofollis liminatans (98.8% identity), (ii) fermentation to propionate by Propionivibrio pelophilus strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol −1 (0.71 mg mg −1 ) and a propionate purity of 80–97% and (iii) acetate production coupled to sulfate reduction by Desulfolutivibrio sulfoxidireducens (98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers. Key points Versatility of anaerobic cultures was explored for waste glycerol valorization Different environmental conditions lead to metabolic specialization Biocommodities such as propionate, acetate and methane were produced