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Biological evaluation of critical bone defect regeneration using hydroxyapatite/ alginate composite granules

ABSTRACT Purpose: to evaluate biocompatibility and osteogenic potential of hydroxyapatite/alginate composite after its implantation on rat calvarian critical bone defect. Methods: thirty adults male Wistar rats were randomly distributed into two groups: GHA – critical bone defect filled with hydroxy...

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Published in:Acta Cirúrgica Brasileira 2024-01, Vol.39
Main Authors: Ribeiro, Iorrana Índira dos Anjos, Almeida, Renata dos Santos, Silva, Ana Maria Guerreiro Braga da, Barbosa Júnior, Aryon de Almeida, Rossi, Alexandre Malta, Miguel, Fúlvio Borges, Rosa, Fabiana Paim
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container_title Acta Cirúrgica Brasileira
container_volume 39
creator Ribeiro, Iorrana Índira dos Anjos
Almeida, Renata dos Santos
Silva, Ana Maria Guerreiro Braga da
Barbosa Júnior, Aryon de Almeida
Rossi, Alexandre Malta
Miguel, Fúlvio Borges
Rosa, Fabiana Paim
description ABSTRACT Purpose: to evaluate biocompatibility and osteogenic potential of hydroxyapatite/alginate composite after its implantation on rat calvarian critical bone defect. Methods: thirty adults male Wistar rats were randomly distributed into two groups: GHA – critical bone defect filled with hydroxyapatite/alginate composite granules (HA/Alg) and CG – critical bone defect without biomaterial; evaluated at biological points of 15, 45 and 120 days. Results: the histomorphometrically analyses for GHA showed osteoid matrix deposition (OM) among the granules and towards the center of the defect in centripetal direction throughout the study, with evident new bone formation at 120 days, resulting in filling 4/5 of the initial bone defect. For CG, this finding was restricted to the edges of the bone margins and formation of connective tissue on the residual area was found in all biological points. Inflammatory response on GHA was chronic granulomatous type, discrete and regressive for all biological points. Throughout the study, the CG presented mononuclear inflammatory infiltrate diffuse and regressive. Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p > 0.05). Conclusions: the biomaterial evaluated at this study showed to be biocompatible, bioactive, osteoconductive and biodegradable synchronously with bone formation.
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Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p &gt; 0.05). 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Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p &gt; 0.05). 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subjects Alginate
Biocompatible Materials
Bone Regeneration
Durapatite
Original
Rats
title Biological evaluation of critical bone defect regeneration using hydroxyapatite/ alginate composite granules
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