Exosome Therapy and Photobiomodulation Therapy Regulate mi-RNA 21, 155 Expressions, Nucleus Acetylation and Glutathione in a Polycystic Ovary Oocyte: An In Vitro Study

Polycystic ovary syndrome (PCOS) is a complex condition that can have various symptoms and complications, one of which is infertility. Dysregulation of miRNA has been associated with the pathogenesis of numerous illnesses such as PCOS. In this study, we evaluated the effect of photobiomodulation the...

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Published in:Journal of lasers in medical sciences 2024-04, Vol.15, p.e10
Main Authors: Sahraeian, Samira, Abbaszadeh, Hojjat Allah, Taheripanah, Robabeh, Edalatmanesh, Mohammad Amin, Keshavarzi, Somayeh, Ghazifard, Alaleh
Format: Article
Language:English
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Summary:Polycystic ovary syndrome (PCOS) is a complex condition that can have various symptoms and complications, one of which is infertility. Dysregulation of miRNA has been associated with the pathogenesis of numerous illnesses such as PCOS. In this study, we evaluated the effect of photobiomodulation therapy (PBMT) and exosome therapy (EXO) on the regulation of miRNA and nucleus acetylation in a PCOS oocyte. In this research, 36 oocytes divided into three groups: control, EXO, and PBM (Wavelength of 640 nm). Subsequently, in-vitro maturation (IVM) was evaluated. Real-time PCR was used to evaluate and . Afterward, oocyte glutathione (GSH) and nucleus acetylation were measured by H4K12. The expression of the and genes in the EXO and PBMT groups was significantly down-regulated in comparison to the control group, but the expression of and significantly increased in the EXO and PBMT groups in comparison to the control group. The EXO and PBMT significantly increased GSH and nucleus acetylation (
ISSN:2008-9783
2228-6721
DOI:10.34172/jlms.2024.10