Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins

Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such as calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in the neck region to facilitate mechano-enzymatic activity. We rec...

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Published in:Journal of experimental botany 2024-04, Vol.75 (8), p.2313-2329
Main Authors: Symonds, Kyle, Teresinski, Howard J, Hau, Bryan, Dwivedi, Vikas, Belausov, Eduard, Bar-Sinai, Sefi, Tominaga, Motoki, Haraguchi, Takeshi, Sadot, Einat, Ito, Kohji, Snedden, Wayne A
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Actins - metabolism
Arabidopsis - genetics
Arabidopsis - metabolism
Calmodulin - metabolism
Myosin Light Chains - chemistry
Myosin Light Chains - metabolism
Protein Binding
Research Papers
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Summary:Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such as calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in the neck region to facilitate mechano-enzymatic activity. We recently identified Arabidopsis CaM-like (CML) proteins CML13 and CML14 as interactors of proteins containing multiple IQ domains, including a myosin VIII. Here, we demonstrate that CaM, CML13, and CML14 bind the neck region of all four Arabidopsis myosin VIII isoforms. Among CMLs tested for binding to myosins VIIIs, CaM, CML13, and CML14 gave the strongest signals using in planta split-luciferase protein interaction assays. In vitro, recombinant CaM, CML13, and CML14 showed specific, high-affinity, calcium-independent binding to the IQ domains of myosin VIIIs. CaM, CML13, and CML14 co-localized to plasma membrane-bound puncta when co-expressed with red fluorescent protein-myosin fusion proteins containing IQ and tail domains of myosin VIIIs. In vitro actin motility assays using recombinant myosin VIIIs demonstrated that CaM, CML13, and CML14 function as light chains. Suppression of CML13 or CML14 expression using RNA silencing resulted in a shortened-hypocotyl phenotype, similar to that observed in a quadruple myosin mutant, myosin viii4KO. Collectively, our data indicate that Arabidopsis CML13 and CML14 are novel myosin VIII light chains.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/erae031