The effect of muscle ultrastructure on the force, displacement and work capacity of skeletal muscle

• Published in: Journal of the Royal Society interface 2024-05, Vol.21 (214), p.20230658
• Main Authors: Dhawale, Nihav, Labonte, David, Holt, Natalie C
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Animals; Biomechanical Phenomena; Life Sciences–Engineering interface; Models, Biological; Muscle Contraction - physiology; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Muscle, Skeletal - ultrastructure; Myosins - metabolism; Sarcomeres - metabolism; Sarcomeres - physiology; Sarcomeres - ultrastructure
• ISSN: 1742-5662; 1742-5689; 1742-5662
• DOI: 10.1098/rsif.2023.0658

Skeletal muscle powers animal movement through interactions between the contractile proteins, actin and myosin. Structural variation contributes greatly to the variation in mechanical performance observed across muscles. In vertebrates, gross structural variation occurs in the form of changes in the muscle cross-sectional area : fibre length ratio. This results in a trade-off between force and displacement capacity, leaving work capacity unaltered. Consequently, the maximum work per unit volume-the work density-is considered constant. Invertebrate muscle also varies in muscle ultrastructure, i.e. actin and myosin filament lengths. Increasing actin and myosin filament lengths increases force capacity, but the effect on muscle fibre displacement, and thus work, capacity is unclear. We use a sliding-filament muscle model to predict the effect of actin and myosin filament lengths on these mechanical parameters for both idealized sarcomeres with fixed actin : myosin length ratios, and for real sarcomeres with known filament lengths. Increasing actin and myosin filament lengths increases stress without reducing strain capacity. A muscle with longer actin and myosin filaments can generate larger force over the same displacement and has a higher work density, so seemingly bypassing an established trade-off. However, real sarcomeres deviate from the idealized length ratio suggesting unidentified constraints or selective pressures.