Early stress exposure on zebrafish development: effects on survival, malformations and molecular alterations

• Published in: Fish physiology and biochemistry 2024-08, Vol.50 (4), p.1545-1562
• Main Authors: Valcarce, David G., Sellés-Egea, Alba, Riesco, Marta F., De Garnica, María-Gracia, Martínez-Fernández, Beatriz, Herráez, María Paz, Robles, Vanesa
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Aquaculture; Biological effects; Biomedical and Life Sciences; Cartilage; Cartilage - abnormalities; Danio rerio; Developmental biology; Developmental stages; Edema; Exposure; Eye; Fish; Fish larvae; Freshwater & Marine Ecology; Gene Expression Regulation, Developmental; Hatching; Histology; Larva - genetics; Larva - growth & development; Larvae; Life Sciences; Malformations; MicroRNAs - genetics; MicroRNAs - metabolism; Morphology; Otoliths; Reproduction; Stress, Physiological; Survival; Swim bladder; Vertebrates; Zebrafish; Zebrafish - genetics; Zoology
• ISSN: 0920-1742; 1573-5168; 1573-5168
• DOI: 10.1007/s10695-024-01355-0

The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health of adult fish and their reproductive performance and overall production. We examined the consequences of direct exposure to successive acute stressors during early development, including their effects on miR-29a and its targets, survival, hatching and malformation rates, larval behaviour and cartilage and eye development. Our aim was to shed light on the pleiotropic effects of early-induced stress in this vertebrate model species. Our results showed that direct exposure to successive acute stressors during early development significantly upregulated miR-29a and downregulated essential collagen transcripts col2a1a , col6a2 and col11a1a , decreased survival and increased malformation rates (swim bladder, otoliths, cardiac oedema and ocular malformations), promoting higher rates of immobility in larvae. Our results revealed that stress in early stages can induce different eye tissular architecture and cranioencephalic cartilage development alterations. Our research contributes to the understanding of the impact of stressful conditions during the early stages of zebrafish development, serving as a valuable model for vertebrate research. This holds paramount significance in the fields of developmental biology and aquaculture and also highlights miR-29a as a potential molecular marker for assessing novel larval rearing programmes in teleost species.