The NADPH-oxidase-associated H+ channel is opened by arachidonate

The H+ channel associated with the generation of O2.- by NADPH oxidase and the oxidase itself must both be activated in response to stimuli (e.g. phorbol esters, chemotactic peptides, certain fatty acids). We have investigated the effects of membrane potential, an imposed pH gradient and a combinati...

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Bibliographic Details
Published in:Biochemical journal 1992-04, Vol.283 (1), p.171-175
Main Authors: HENDERSON, L. M, CHAPELL, J. B
Format: Article
Language:English
Subjects:
Arachidonic Acid - pharmacology
Biological and medical sciences
Carrier Proteins - physiology
Cell Membrane Permeability - drug effects
Cell physiology
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen-Ion Concentration
Ion Channel Gating - drug effects
Ion Channels - drug effects
Ion Channels - physiology
Liposomes - metabolism
Membrane and intracellular transports
Membrane Potentials - physiology
Molecular and cellular biology
NADH, NADPH Oxidoreductases - physiology
NADPH Oxidases
Potassium-Hydrogen Antiporters
Superoxides - metabolism
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Summary:The H+ channel associated with the generation of O2.- by NADPH oxidase and the oxidase itself must both be activated in response to stimuli (e.g. phorbol esters, chemotactic peptides, certain fatty acids). We have investigated the effects of membrane potential, an imposed pH gradient and a combination of the two (the protonmotive force) on the H+ conductivity of the cytoplast membrane. H+ conductivity was observed only in the presence of arachidonate and not in its absence. In the presence of arachidonate, H+ movement was determined by the protonmotive force. The effect of arachidonate was probably on a channel, since this fatty acid did not significantly increase the H+ permeability of artificial phospholipid membranes. It appears, therefore, that arachidonate is required both for the activation of O2.- production and the associated H(+)-channel-mediated efflux.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2830171