Structural and mechanistic conservation in DNA ligases

DNA ligases are enzymes required for the repair, replication and recombination of DNA. DNA ligases catalyse the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA. Despite their occurrence in all organisms, DNA ligases show a wide diversity of amino acid sequences, mole...

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Bibliographic Details
Published in:Nucleic acids research 2000-11, Vol.28 (21), p.4051-4058
Main Authors: Doherty, A J, Suh, S W
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Animals
Binding Sites
Catalysis
Conserved Sequence
DNA Ligases - chemistry
DNA Ligases - classification
DNA Ligases - metabolism
Humans
Models, Molecular
Molecular Sequence Data
NAD
NAD - metabolism
Protein Structure, Tertiary
Substrate Specificity
Survey and Summary
Zinc Fingers
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Summary:DNA ligases are enzymes required for the repair, replication and recombination of DNA. DNA ligases catalyse the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA. Despite their occurrence in all organisms, DNA ligases show a wide diversity of amino acid sequences, molecular sizes and properties. The enzymes fall into two groups based on their cofactor specificity, those requiring NAD(+) for activity and those requiring ATP. The eukaryotic, viral and archael bacteria encoded enzymes all require ATP. NAD(+)-requiring DNA ligases have only been found in prokaryotic organisms. Recently, the crystal structures of a number of DNA ligases have been reported. It is the purpose of this review to summarise the current knowledge of the structure and catalytic mechanism of DNA ligases.
ISSN:1362-4962
0305-1048
1362-4962
DOI:10.1093/nar/28.21.4051