Expression of the CD15 differentiation antigen (3-fucosyl-N-acetyl-lactosamine, LeX) on putative neutrophil adhesion molecules CR3 and NCA-160

The expression of the carbohydrate antigen 3-fucosyl-N-acetyl-lactosamine (CD15, LeX) on human neutrophil glycoproteins has been studied by immunoprecipitation and immunoblotting by using monoclonal antibody MC2. The antigen is expressed on membrane glycoproteins of approximate molecular mass 165 an...

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Bibliographic Details
Published in:Biochemical journal 1990-06, Vol.268 (2), p.275-280
Main Authors: Stocks, S C, Albrechtsen, M, Kerr, M A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antigens, CD - immunology
Antigens, Differentiation - immunology
Antigens, Differentiation, Myelomonocytic - immunology
Antigens, Neoplasm
Cell Adhesion Molecules
Cell Membrane - immunology
Complement C3b - immunology
Epitopes - immunology
Glycoproteins - immunology
Humans
Lewis X Antigen
Lymphocyte Activation
Mice
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Neutrophils - drug effects
Neutrophils - immunology
Receptors, Complement - immunology
Receptors, Complement 3b
Trisaccharides - metabolism
Up-Regulation
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Summary:The expression of the carbohydrate antigen 3-fucosyl-N-acetyl-lactosamine (CD15, LeX) on human neutrophil glycoproteins has been studied by immunoprecipitation and immunoblotting by using monoclonal antibody MC2. The antigen is expressed on membrane glycoproteins of approximate molecular mass 165 and 105 kDa. These glycoproteins include the complement receptor and adhesion molecule, CR3, in which the beta-chain (CD18, 105 kDa) shows much greater expression than the alpha-chain (CD11b, 165 kDa). Most of the 165 kDa CD15 antigen is accounted for by expression on the carcinoembryonic antigen (CEA)-related molecule NCA160. Other members of this family, NCA95, NCA90 and NCA55, which are also found in neutrophils, do not express the CD15 antigen. There is a marked increase in the surface expression of CD15, CR3 and the antigen recognized by anti-CEA antibodies upon activation of neutrophils by the chemotactic peptide N-formylmethionyl-leucylphenylalanine.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2680275